The interaction between emodin, frangulin-A (obtained from Ventilago leiocarpa Benth) with bovine serum albumin (BSA), and calf thymus DNA (ct-DNA) has been investigated. The fluorescence spectrum and molecular docking methods are used to investigate the interaction between emodin, frangulin-A, and BSA. The UV spectrum revealed interaction between emodin, frangulin-A, and ct-DNA. The fluorescence spectrum confirmed that the compounds of emodin–BSA and frangulin-A–BSA were formed showing fl uorescence quenching and a decrease of the maximum emission wavelength. The simulated molecular docking showed stable combinations of emodin–BSA and frangulin-A– BSA. Thus, molecular interactions mainly occur due to hydrophobic forces and hydrogen bonds. According to the UV spectrum, emodin and frangulin-A interacted with ct-DNA via electrostatic interaction.

中文翻译：

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大黄素及其衍生物Frangulin-A与牛血清白蛋白和小牛胸腺DNA的相互作用

已经研究了大黄素，乳清蛋白-A（从Leocarpa Benth获得）与牛血清白蛋白（BSA）和小牛胸腺DNA（ct-DNA）之间的相互作用。荧光光谱和分子对接方法用于研究大黄素，乳腺素A和BSA之间的相互作用。紫外光谱揭示了大黄素，促性腺素A和ct-DNA之间的相互作用。荧光光谱证实形成了大黄素-BSA和霜蛋白-A-BSA化合物，显示出荧光猝灭和最大发射波长的减小。模拟的分子对接显示大黄素-BSA和乳蛋白-A-BSA的稳定组合。因此，分子相互作用主要由于疏水力和氢键而发生。根据紫外光谱，大黄素和乳蛋白-A通过静电相互作用与ct-DNA相互作用。