In recent decades, many new flavi-like viruses have been discovered predominantly in different invertebrates and, as was recently shown, some of them may cause disease in humans. The Jingmenvirus (JMV) group holds a special place among flaviviruses and flavi-like viruses because they have a segmented ssRNA(+) genome. We detected Alongshan virus (ALSV), which is a representative of the JMV group, in ten pools of adult Ixodes persulcatus ticks collected in two geographically-separated Russian regions. Three of the ten strains were isolated in the tick cell line IRE/CTVM19. One of the strains persisted in the IRE/CTVM19 cells without cytopathic effect for three years. Most ALSV virions purified from tick cells were spherical with a diameter of approximately 40.5 nm. In addition, we found smaller particles of approximately 13.1 nm in diameter. We obtained full genome sequences of all four segments of two of the isolated ALSV strains, and partial sequences of one segment from the third strain. Phylogenetic analysis on genome segment 2 of the JMV group clustered our novel strains with other ALSV strains. We found evidence for the existence of a novel upstream open reading frame in the glycoprotein-coding segment of ALSV and other members of the JMV group.

中文翻译：

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俄罗斯阿隆山病毒的分离与鉴定。

近几十年来，主要在不同的无脊椎动物中发现了许多新的类似黄病毒的病毒，正如最近显示的那样，其中一些可能会导致人类疾病。荆门病毒 (JMV) 组在黄病毒和类黄病毒中占有特殊的位置，因为它们具有分段的 ssRNA(+) 基因组。我们在两个地理上分隔的俄罗斯地区收集的十个成年全长硬蜱蜱池中检测到了作为 JMV 组代表的阿隆山病毒 (ALSV)。在蜱细胞系 IRE/CTVM19 中分离出十种菌株中的三种。其中一种菌株在 IRE/CTVM19 细胞中持续存在三年而没有细胞病变效应。从蜱细胞中纯化的大多数 ALSV 病毒粒子是直径约为 40.5 nm 的球形。此外，我们还发现了直径约为 13.1 nm 的较小颗粒。我们获得了两个分离的 ALSV 菌株的所有四个片段的完整基因组序列，以及来自第三个菌株的一个片段的部分序列。对 JMV 组基因组片段 2 的系统发育分析将我们的新菌株与其他 ALSV 菌株聚集在一起。我们发现在 ALSV 和 JMV 组的其他成员的糖蛋白编码片段中存在新的上游开放阅读框的证据。