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Chemical structure and effects of antioxidation and against α-glucosidase of natural polysaccharide from Glycyrrhiza inflata Batalin.
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-03-26 , DOI: 10.1016/j.ijbiomac.2020.03.192 Li-Chao Pan 1 , Yong-Ming Zhu 1 , Zhen-Yuan Zhu 1 , Wei Xue 1 , Chun-Yu Liu 1 , Hui-Qing Sun 1 , Yue-Yin 1
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-03-26 , DOI: 10.1016/j.ijbiomac.2020.03.192 Li-Chao Pan 1 , Yong-Ming Zhu 1 , Zhen-Yuan Zhu 1 , Wei Xue 1 , Chun-Yu Liu 1 , Hui-Qing Sun 1 , Yue-Yin 1
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GIBP, a homogeneous polysaccharide extracted from Glycyrrhiza inflata Batalin with a molecular weight 1.96 × 103 kDa, had a triple helix structure, smooth and sheet-like structure. Comprehensive analysis showed that the main chain of GIBP was composed of α-D-1,4 linked glucose, branch points were composed of α-D-1,3,6 and α-D-1,2,3,6 linked glucoses, and side chains were composed of α-D-1,3 and β-D-1,6 linked galactose, β-L-1,2 linked arabinose, α-D-1,3 and β-D-1,3 mannose. The scavenging abilities of GIBP (3 mg/mL) against DPPH radical, OH radical, O2- radical and ABTS were 50.75 ± 0.13% and 52.32 ± 0.13, 25.84 ± 0.35% and 44.57 ± 0.15% and it also demonstrated an obvious dose-effect relationship. The inhibitory activity of α-glucosidase showed that the inhibitory effect of GIBP was enhanced with the increase of concentration. When the concentration reached 6 mg/mL, the inhibition rate of α-glucosidase activity reached 64.77%. And the ka, kd and KD were 6.472 × 104 1/Ms., 2.934 × 10-3 1/s and 4.534 × 10-8 M.
中文翻译：
甘草天然巴塔林天然多糖的化学结构及抗氧化和抗α-葡萄糖苷酶的作用。
GIBP是一种从大豆甘草中提取的均质多糖，分子量为1.96×103 kDa，具有三重螺旋结构，光滑且呈片状结构。综合分析表明，GIBP的主链由α-D-1,4连接的葡萄糖组成，分支点由α-D-1,3,6和α-D-1,2,3,6连接的葡萄糖组成，侧链由α-D-1,3和β-D-1,6连接的半乳糖，β-L-1,2连接的阿拉伯糖，α-D-1,3和β-D-1,3组成甘露糖。GIBP（3 mg / mL）对DPPH自由基，OH自由基，O2-自由基和ABTS的清除能力分别为50.75±0.13％和52.32±0.13、25.84±0.35％和44.57±0.15％，并且还显示出明显的剂量-效果关系。α-葡萄糖苷酶的抑制活性表明，GIBP的抑制作用随浓度的增加而增强。当浓度达到6 mg / mL时，α-葡萄糖苷酶活性的抑制率达到64.77％。ka，kd和KD分别为6.472×104 1 / Ms，2.934×10-3 1 / s和4.534×10-8 M.
更新日期：2020-03-27
中文翻译：
甘草天然巴塔林天然多糖的化学结构及抗氧化和抗α-葡萄糖苷酶的作用。
GIBP是一种从大豆甘草中提取的均质多糖，分子量为1.96×103 kDa，具有三重螺旋结构，光滑且呈片状结构。综合分析表明，GIBP的主链由α-D-1,4连接的葡萄糖组成，分支点由α-D-1,3,6和α-D-1,2,3,6连接的葡萄糖组成，侧链由α-D-1,3和β-D-1,6连接的半乳糖，β-L-1,2连接的阿拉伯糖，α-D-1,3和β-D-1,3组成甘露糖。GIBP（3 mg / mL）对DPPH自由基，OH自由基，O2-自由基和ABTS的清除能力分别为50.75±0.13％和52.32±0.13、25.84±0.35％和44.57±0.15％，并且还显示出明显的剂量-效果关系。α-葡萄糖苷酶的抑制活性表明，GIBP的抑制作用随浓度的增加而增强。当浓度达到6 mg / mL时，α-葡萄糖苷酶活性的抑制率达到64.77％。ka，kd和KD分别为6.472×104 1 / Ms，2.934×10-3 1 / s和4.534×10-8 M.
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