Determination of the capsular polysaccharide structure of the Klebsiella pneumoniae ST512 representative strain KPB-1 and assignments of the glycosyltransferases functions.,International Journal of Biological Macromolecules

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Determination of the capsular polysaccharide structure of the Klebsiella pneumoniae ST512 representative strain KPB-1 and assignments of the glycosyltransferases functions.
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-03-26 , DOI: 10.1016/j.ijbiomac.2020.03.196
Barbara Bellich ₁ , Cristina Lagatolla ₁ , Roberto Rizzo ₁ , Marco Maria D'Andrea ₂ , Gian Maria Rossolini ₃ , Paola Cescutti ₁

Affiliation

Klebsiella pneumoniae strain KPB-1 was isolated in early 2011 from the pleural fluid of an inpatient admitted at an Italian hospital. It was characterized to produce the KPC-3 carbapenemase and to belong to sequence type 512, a derivative of sequence type 258 clade II characterized by the cps-2 gene cluster. The K-antigen of K. pneumoniae KPB-1 was purified and its structure determined by using GLC-MS of appropriate carbohydrate derivatives and 1D and 2D NMR spectroscopy of the native polysaccharide. All the collected data demonstrated the following repeating unit for the K. pneumoniae KPB-1 capsular polysaccharide: The reactions catalysed by each glycosyltransferase in the cps-2 gene cluster were assigned on the basis of structural homology with other Klebsiella K antigens.

中文翻译：

肺炎克雷伯氏菌ST512代表性菌株KPB-1荚膜多糖结构的确定以及糖基转移酶功能的确定。

2011年初，从一家意大利医院的住院病人的胸膜液中分离出肺炎克雷伯菌KPB-1株。其特征在于产生KPC-3碳青霉烯酶并属于序列类型512，序列类型258是进化枝Ⅱ的衍生物，其特征在于cps-2基因簇。纯化肺炎克雷伯氏菌KPB-1的K抗原，并通过使用适当的碳水化合物衍生物的GLC-MS以及天然多糖的1D和2D NMR光谱法确定其结构。所有收集的数据均证明肺炎克雷伯菌KPB-1荚膜多糖具有以下重复单元：cps-2基因簇中每个糖基转移酶催化的反应均基于与其他克雷伯菌K抗原的结构同源性进行分配。

更新日期：2020-03-27

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