Thyroglobulin (Tg) is an important index for the surveillance of differentiated thyroid carcinoma, and yet sensitive and specific protein detection still faces challenges in both biomedical research and clinical diagnosis. We described a novel approach to synthesize molecular imprinting polymers (MIPs)/hemin-graphene nanosheets (H-GNs) composites on the paper, using Tg and 3,3′,5,5′-tetramethylbenzidine (TMB, probe) as double templates. H-GNs with intrinsic properties can initiate the free-radical polymerization to prepare MIPs and catalyze the coloration of peroxidase substrates such as TMB for colorimetric detection. Template-probe double imprinting strategy enhanced the accessibility of the probe to the catalytic interface without interfering the specific recognition of template molecule. Under the optimized conditions, the gray intensity was proved to be proportional to the concentration of Tg in the range of 0.7-100 ng mL^-1 with a detection limit of 0.1 ng mL^-1 (1.5 fM). Anti-Tg antibodies and sample matrix did not affect the determination of the target protein, nor did they enhance the background noise. The use of paper-based device exhibited the advantages of low cost, easy handling and portability. It was supposed to be applied for Tg determination in serum to evaluate persistent or recurrent differentiated thyroid carcinoma.

甲状腺球蛋白（Tg）是监测分化型甲状腺癌的重要指标，但是敏感和特异性蛋白质检测在生物医学研究和临床诊断中仍然面临挑战。我们在纸上描述了一种新颖的方法来合成分子印迹聚合物（MIP）/血红素石墨烯纳米片（H-GNs）复合材料，使用Tg和3,3'，5,5'-四甲基联苯胺（TMB，探针）作为双模板。具有内在特性的H-GNs可以引发自由基聚合反应以制备MIP，并催化过氧化物酶底物（如TMB）的显色，用于比色检测。模板探针双印迹策略可增强探针接近催化界面的可及性，而不会干扰模板分子的特异性识别。在优化条件下^-1的检出限为0.1 ng mL ^-1（1.5 fM）。抗Tg抗体和样品基质既不影响目标蛋白的测定，也不增强背景噪音。纸基设备的使用具有成本低，易于处理和便携性的优点。它被认为可用于血清中Tg的测定，以评估持续或复发分化的甲状腺癌。