Demand for omega-3 long chain polyunsaturated fatty acids has become global challenge for aquaculture and different components have been used to increase nutritional value of fillets. The aim of this study was to evaluate influences of feeds on zootechnical parameters, biochemical plasma parameters, expression of lipid-dependent genes, hepatocyte histomorphologies, and fatty acid profiles in common carp fillets. We compared a control diet (CTRL), mimicking a commercial feed formulation for common carp, with three diets containing blends of vegetable oils and a DHA-rich alga (Schizochytrium sp.) included at 3.125% (CB1) or 1.563% (CB2), and 2.1% salmon oil (CB3). The study revealed no differences in final body weight of fish fed CB1-3 diets in comparison with significantly lower CTRL. Concentrations of all biochemical parameters in plasma increased gradually in fish fed CB1-3 diets when compared to CTRL diet, with exception of triacylglycerol levels. Expression of hepatic fas, elovl-5a and pparα genes increased significantly in fish fed CB1 and CB2. Additionally, eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) accumulation in muscle tissue was directly proportional to the amounts supplied in the diets. Our study revealed that carp fillet profiles can be manipulated for DHA and EPA-contents using enriched diets, depending on the source of fat.

中文翻译：

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饲喂不同饮食脂肪来源的鲤鱼的血浆生化，基因表达和肝组织形态学。

对omega-3长链多不饱和脂肪酸的需求已成为水产养殖的全球性挑战，并且已使用不同的成分来提高鱼片的营养价值。这项研究的目的是评估饲料对鲤鱼鱼片中动物技术参数，生化血浆参数，脂质依赖性基因表达，肝细胞组织形态和脂肪酸谱的影响。我们比较了模仿普通鲤鱼商业饲料配方的对照饮食（CTRL）和包含植物油和富含DHA的藻类（Schizochytrium sp。）的三种饮食的混合比例，分别为3.125％（CB1）或1.563％（CB2） ，以及2.1％的鲑鱼油（CB3）。该研究表明，饲喂CB1-3日粮的鱼的最终体重与CTRL明显降低相比没有差异。与CTRL饲料相比，饲喂CB1-3饲料的鱼血浆中所有生化参数的浓度逐渐增加，但三酰甘油水平除外。喂食CB1和CB2的鱼肝fas，lovlov-5a和pparα基因的表达显着增加。另外，二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）在肌肉组织中的积累与饮食中的供应量成正比。我们的研究表明，根据脂肪的来源，可以使用丰富的饮食控制鲤鱼鱼片的DHA和EPA含量。二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）在肌肉组织中的积累与饮食中提供的数量成正比。我们的研究表明，根据脂肪的来源，可以使用丰富的饮食控制鲤鱼鱼片的DHA和EPA含量。二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）在肌肉组织中的积累与饮食中提供的数量成正比。我们的研究表明，根据脂肪的来源，可以使用丰富的饮食控制鲤鱼鱼片的DHA和EPA含量。