Two Lytic polysaccharide Mono-Oxygenases (LPMOs), non-modular (PMO_08942) and modular (PMO_07920), from thermotolerant fungus Aspergillus terreus 9DR cloned and expressed in Pichia pastoris X33 and purified to homogeneity using ion-exchange chromatography were found to be of ~29 and ~40 kDa, respectively. Both LPMOs were optimally active at 50 °C; PMO_08942 was active under acidic condition (pH 5.0) and PMO_07920 at pH 7.0. Modular LPMO (PMO_07920) tethered to CBM-1 was found to be versatile as it showed appreciable activity on complex polysaccharide (both cellulose and xylans) as compared to non-modular (PMO_08942). The t1/2 of PMO_08942 (~192 h, pH 5.0) and PMO_0792 (~192 h, pH 7.0) at 50 °C, suggests highly stable nature of these LPMOs. Fluorescently tagged modular AA9 was studied microscopically to understand interaction with pretreated biomass. Priming of biomass for up to 6 h with LPMOs prior to initiating hydrolysis with core cellulase enzyme resulted in significantly higher saccharification.

中文翻译：

---

用糖基水解酶糖化之前用裂解多糖单加氧酶引发木质纤维素的创新方法可以节省第二代乙醇工艺。

发现来自耐热真菌曲霉曲霉9DR的两种非模块化（PMO_08942）和模块化（PMO_07920）的溶菌多糖单加氧酶（LPMO）在毕赤酵母X33中克隆并表达，并使用离子交换层析纯化至同质分别为29和40 kDa。两种LPMO在50°C时均具有最佳活性。PMO_08942在酸性条件下（pH 5.0）具有活性，而PMO_07920在pH 7.0下具有活性。系留在CBM-1上的模块化LPMO（PMO_07920）具有多种用途，因为与非模块化（PMO_08942）相比，它对复杂的多糖（纤维素和木聚糖）表现出明显的活性。在50°C下PMO_08942（〜192 h，pH 5.0）和PMO_0792（〜192 h，pH 7.0）的t1 / 2表明这些LPMO的高度稳定性。显微镜下研究了荧光标记的模块化AA9，以了解与预处理生物质的相互作用。在开始用核心纤维素酶水解之前，用LPMO引发生物质长达6小时，导致糖化明显提高。