Abstract In this study, the impact of high oxygen atmospheric packaging (HOAP) on hydrogen peroxide (H2O2) production, and on the content of hemicellulose, cellulose, lignin, and protein accumulation in Chinese flowering cabbage (Brassica campestris L. ssp. chinensis Makino) was assessed by using air packages as a control. The results showed that HOAP efficiently reduced the H2O2 levels in the stem of Chinese flowering cabbage during the storage process. An increase in tissue firmness, and significant reduction in the content of hemicellulose, cellulose, and lignin in the stem were observed upon HOAP treatment. The activities of peroxidases (POD) and laccases were also suppressed. Furthermore, proteomic profiling revealed a total of 63 differentially expressed proteins, of which oxidative stress-related proteins (enzymes) were found to be the major proteins that were regulated by HOAP treatment. The reduction in hemicellulose, cellulose, and lignin biosynthesis regulated by H2O2 signal was probably related to the differential accumulation of oxidative stress-related proteins that were induced by the HOAP treatment.

中文翻译：

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高氧大气包装（HOAP）通过调节大白菜氧化应激相关蛋白的积累来减少H2O2的产生

摘要 在这项研究中，高氧常压包装 (HOAP) 对过氧化氢 (H2O2) 生产的影响，以及对大白菜 (Brassica campestris L. ssp. chinensis Makino) 中半纤维素、纤维素、木质素和蛋白质积累的影响。 ) 是通过使用空气包作为对照进行评估的。结果表明，在贮藏过程中，HOAP 有效降低了大白菜茎中的 H2O2 含量。在 HOAP 处理后，观察到组织硬度增加，茎中半纤维素、纤维素和木质素的含量显着降低。过氧化物酶 (POD) 和漆酶的活性也受到抑制。此外，蛋白质组学分析揭示了总共 63 种差异表达的蛋白质，其中氧化应激相关蛋白（酶）被发现是受 HOAP 处理调节的主要蛋白质。受 H2O2 信号调节的半纤维素、纤维素和木质素生物合成的减少可能与 HOAP 处理诱导的氧化应激相关蛋白的差异积累有关。