PtCuCo trimetallic alloys (PtCuCo-TAs) are synthesized by one-step reduction. The chemiluminescence (CL) properties of PtCuCo-TAs are studied systemically. PtCuCo-TAs show good catalyzing for luminol-H2O2 system. A CL platform is developed for the detection of miRNA-21 using PtCuCo-TAs as nanozyme catalyzer. In the CL detection platform, H1 (Hairpin DNA1) is immobilized onto magnetic beads (MBs) firstly. In the presence of miRNA-21, H1 is opened. H2 (Hairpin DNA2) then hybridizes with H1. Meanwhile, a "cleat" in the end of miRNA-21 with a fewer bases complementary is formed to prevent miRNA-21 dissociating from H1. This miRNA-21 hybridizes to another H1. When cpDNA-PtCuCo-TAs which consisted with cDNA (Complementary strand of probe DNA) and pDNA-PtCuCo-TAs (PtCuCo-TAs labeled with probe DNA) are added, the ssDNA region of H1 reacts with the toehold domain of probe DNA and cDNA is released resulting pDNA-PtCuCo-TAs being captured. With this process repeatedly, a lot of pDNA-PtCuCo-TAs are captured onto MBs. After separation and washing, the precipitate and H2O2 are put into the 96-well and luminol solution is injected. The CL signal is produced by PtCuCo-TAs catalyzing luminol-H2O2 system. The amount of miRNA-21 is detected with CL signal. This CL platform performs with limit of detection 0.167 fM and has good selectivity over other RNA.

中文翻译：

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用于鲁米诺-H2O2化学发光的三金属合金纳米酶催化剂的一步制备及其与miRNA步行机结合在miRNA-21检测中的应用。

通过一步还原合成PtCuCo三金属合金（PtCuCo-TAs）。系统研究了PtCuCo-TA的化学发光（CL）特性。PtCuCo-TAs对鲁米诺-H2O2系统显示出良好的催化作用。开发了一个CL平台，用于使用PtCuCo-TAs作为纳米酶催化剂检测miRNA-21。在CL检测平台中，首先将H1（Hairpin DNA1）固定在磁珠（MBs）上。在存在miRNA-21的情况下，打开H1。H2（发夹DNA2）然后与H1杂交。同时，在miRNA-21末端形成了具有较少碱基互补性的“切割”，以防止miRNA-21从H1解离。该miRNA-21与另一个H1杂交。当添加由cDNA（探针DNA的互补链）组成的cpDNA-PtCuCo-TA和pDNA-PtCuCo-TA（被探针DNA标记的PtCuCo-TA）组成时，H1的ssDNA区域与探针DNA的前额域反应，释放出cDNA，从而捕获了pDNA-PtCuCo-TA。反复进行此过程，许多pDNA-PtCuCo-TA被捕获到MB上。分离并洗涤后，将沉淀物和H2O2放入96孔中，并注入鲁米诺溶液。CL信号是由PtCuCo-TAs催化Luminol-H2O2系统产生的。用CL信号检测miRNA-21的量。该CL平台的检测极限为0.167 fM，对其他RNA具有良好的选择性。CL信号是由PtCuCo-TAs催化Luminol-H2O2系统产生的。用CL信号检测miRNA-21的量。该CL平台的检测极限为0.167 fM，对其他RNA具有良好的选择性。CL信号是由PtCuCo-TAs催化Luminol-H2O2系统产生的。用CL信号检测miRNA-21的量。该CL平台的检测极限为0.167 fM，对其他RNA具有良好的选择性。