A new amino-modified Scholl-coupling mesoporous polymer (NH2@SMPA)-online solid-phase extraction method, coupled with high-performance liquid chromatography (online SPE-HPLC) was established for the analysis of six plant growth regulators (PGRs) in bean sprouts. NH2@SMPA was synthesized by acid-catalyzed deacetylation of acetylamino-Scholl-coupling mesoporous polymer (SMPA). The diversity of functional groups, such as aromatic, acetylamino, and NH2, was conducive to multiple binding interactions between NH2@SMPA and PGRs. NH2@SMPA exhibited superior extraction capability for PGRs, compared with SMPA and commercial adsorbents. The extraction conditions, including loading solvent, pH of loading solution, eluting solvent, and flow rates of loading and elution, were optimized. Under the optimized conditions, wide linear ranges (0.01-500 μg kg-1) and low detection limits (2.34-20.2 ng kg-1) were obtained. The recoveries were satisfactory, i.e., 86.0% to 109% with relative standard deviations ≤9.8% (n = 3). Finally, the online SPE-HPLC method was successfully used for determination of PGRs in bean sprouts.

中文翻译：

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氨基改性的Scholl偶联介孔聚合物，用于从豆芽中在线固相提取植物生长调节剂。

建立了一种新的氨基修饰的Scholl偶联中孔聚合物（NH2 @ SMPA）在线固相萃取方法，并结合了高效液相色谱（在线SPE-HPLC）用于分析6种植物生长调节剂（PGRs）。豆芽。NH2 @ SMPA是通过酸催化乙酰氨基-Scholl偶联介孔聚合物（SMPA）的脱乙酰作用合成的。芳香族，乙酰氨基和NH2等官能团的多样性有助于NH2 @ SMPA和PGR之间的多重结合相互作用。与SMPA和市售吸附剂相比，NH2 @ SMPA对PGR表现出优异的提取能力。优化了萃取条件，包括加载溶剂，加载溶液的pH，洗脱溶剂以及加载和洗脱的流速。在优化条件下，线性范围宽（0。获得了01-500μgkg-1）和低检测限（2.34-20.2 ng kg-1）。回收率令人满意，即86.0％至109％，相对标准偏差≤9.8％（n = 3）。最后，在线SPE-HPLC方法已成功用于豆芽中PGR的测定。