Real-time monitoring of intracellular pH in live cells with fluorescent ionic liquid,Analytica Chimica Acta

当前位置： X-MOL 学术 › Anal. Chim. Acta › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Real-time monitoring of intracellular pH in live cells with fluorescent ionic liquid
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.050
Lifang Gao ₁ , Xin Lin ₁ , Anqi Zheng ₁ , E Shuang ₁ , Jianhua Wang ₁ , Xuwei Chen ₁

Affiliation

Real-time monitoring of intracellular pH is of great significance due to its essential role in physiological and pathological processes. In present work, the ionic liquid (IL) N-methyl-6-hydroxyquinolinium bis(trifluoromethylsulfonyl) imide ([6MQc][NTf2]) is proposed as a fluorescence probe for the quantitative imaging of intracellular pH in response to external stimuli. The fluorescence of the IL [6MQc][NTf2] exhibits a sensitive response to pH variations, as the deprotonation of [6MQc][NTf2] generates the highly fluorescent zwitterionic product [6MQz]. pH fluctuations in the range of 6.0-7.5 can be accurately sensed by monitoring the fluorescence change at 555 nm. Moreover, this IL probe exhibits favorable biocompatibility, excellent anti-photobleaching properties, and high tolerance to ionic strength. Using the IL probe, real-time sensing of hypoxia- and drug-induced intracellular pH changes in MCF-7 cells is achieved.

中文翻译：

荧光离子液体实时监测活细胞内pH值

由于其在生理和病理过程中的重要作用，实时监测细胞内 pH 值具有重要意义。在目前的工作中，离子液体 (IL) N-甲基-6-羟基喹啉双（三氟甲基磺酰基）亚胺（[6MQc][NTf2]）被提议作为荧光探针，用于响应外部刺激的细胞内 pH 值的定量成像。IL [6MQc][NTf2] 的荧光表现出对 pH 变化的敏感响应，因为 [6MQc][NTf2] 的去质子化会产生高度荧光的两性离子产物 [6MQz]。通过监测 555 nm 处的荧光变化，可以准确感知 6.0-7.5 范围内的 pH 波动。此外，该IL探针具有良好的生物相容性、优异的抗光漂白性能和对离子强度的高耐受性。使用 IL 探针，

更新日期：2020-05-01

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11