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Real-time monitoring of intracellular pH in live cells with fluorescent ionic liquid
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.aca.2020.03.050
Lifang Gao 1 , Xin Lin 1 , Anqi Zheng 1 , E Shuang 1 , Jianhua Wang 1 , Xuwei Chen 1
Affiliation  

Real-time monitoring of intracellular pH is of great significance due to its essential role in physiological and pathological processes. In present work, the ionic liquid (IL) N-methyl-6-hydroxyquinolinium bis(trifluoromethylsulfonyl) imide ([6MQc][NTf2]) is proposed as a fluorescence probe for the quantitative imaging of intracellular pH in response to external stimuli. The fluorescence of the IL [6MQc][NTf2] exhibits a sensitive response to pH variations, as the deprotonation of [6MQc][NTf2] generates the highly fluorescent zwitterionic product [6MQz]. pH fluctuations in the range of 6.0-7.5 can be accurately sensed by monitoring the fluorescence change at 555 nm. Moreover, this IL probe exhibits favorable biocompatibility, excellent anti-photobleaching properties, and high tolerance to ionic strength. Using the IL probe, real-time sensing of hypoxia- and drug-induced intracellular pH changes in MCF-7 cells is achieved.

中文翻译:

荧光离子液体实时监测活细胞内pH值

由于其在生理和病理过程中的重要作用,实时监测细胞内 pH 值具有重要意义。在目前的工作中,离子液体 (IL) N-甲基-6-羟基喹啉双(三氟甲基磺酰基)亚胺([6MQc][NTf2])被提议作为荧光探针,用于响应外部刺激的细胞内 pH 值的定量成像。IL [6MQc][NTf2] 的荧光表现出对 pH 变化的敏感响应,因为 [6MQc][NTf2] 的去质子化会产生高度荧光的两性离子产物 [6MQz]。通过监测 555 nm 处的荧光变化,可以准确感知 6.0-7.5 范围内的 pH 波动。此外,该IL探针具有良好的生物相容性、优异的抗光漂白性能和对离子强度的高耐受性。使用 IL 探针,
更新日期:2020-05-01
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