Abstract Chemically induced anaesthesia is currently the only approved method for anaesthetising fish for sampling and surgical procedures by many organizations, including the Canadian Council on Animal Care, Health Canada, and the U.S. Food and Drug Administration (FDA). Although MS-222 (Tricaine-S) performs well, the long chemical withdrawal time makes it a poor choice for field-based research where fish cannot be held very long, especially in areas where commercial, subsistence or recreational fisheries occur. The lack of approved anaesthetic options for field research is mainly due to a lack of research on the suitability of alternatives, particularly for surgery. In the present study, juvenile brook trout (Salvelinus fontinalis) were subjected to a handling stressor or surgical stressor (tag implantation) using one of three anaesthetics: MS-222, AQUI-S®, and electro-immobilization. Indicators of stress (blood glucose and plasma cortisol) were measured over five time points (0 h, 1 h, 4 h, 24 h, 1 week post-stressor). The results from this study suggest some time-specific differences in stress levels between anaesthetics; however, there was limited evidence to suggest differences in the efficacy of the anaesthetic methods at mediating stress levels in brook trout. Thus, both AQUI-S® and electro-immobilization may provide suitable alternatives to MS-222 for field research.

中文翻译：

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为实地研究寻找麻醉替代方案

摘要 化学诱导麻醉是目前许多组织（包括加拿大动物保健委员会、加拿大卫生部和美国食品和药物管理局 (FDA)）批准的用于麻醉鱼类进行取样和外科手术的唯一方法。尽管 MS-222 (Tricaine-S) 表现良好，但较长的化学品停药时间使其成为不能长时间饲养鱼类的实地研究的糟糕选择，尤其是在商业、自给或休闲渔业发生的地区。缺乏用于实地研究的批准麻醉选择主要是由于缺乏对替代品的适用性的研究，特别是对于手术。在本研究中，使用以下三种麻醉剂中的一种，对幼年鳟鱼 (Salvelinus fontinalis) 进行处理应激源或手术应激源（标签植入）：MS-222、AQUI-S® 和电固定。在五个时间点（0 小时、1 小时、4 小时、24 小时、应激后 1 周）测量压力指标（血糖和血浆皮质醇）。这项研究的结果表明，麻醉剂之间的压力水平存在某些时间特异性差异；然而，有限的证据表明麻醉方法在调节鳟鱼应激水平方面的功效存在差异。因此，AQUI-S® 和电固定可以为现场研究提供合适的 MS-222 替代品。有限的证据表明麻醉方法在调节鳟鱼应激水平方面的功效存在差异。因此，AQUI-S® 和电固定可以为现场研究提供合适的 MS-222 替代品。有限的证据表明麻醉方法在调节鳟鱼应激水平方面的功效存在差异。因此，AQUI-S® 和电固定可以为现场研究提供合适的 MS-222 替代品。