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Genomic and histopathological characteristics of Vibrio parahaemolyticus isolated from an acute hepatopancreatic necrosis disease outbreak in Pacific white shrimp (Penaeus vannamei) cultured in Korea
Aquaculture ( IF 4.5 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.aquaculture.2020.735284 Jee Eun Han , Seong-Kyoon Choi , Se-Hyeon Han , Seung Chan Lee , Hye Jin Jeon , Chorong Lee , Kyeong Yeon Kim , Young Seo Lee , Seul Chan Park , Gahngyoon Rhee , Seon Young Park , Jun-Seob Kim , Song Park , Ji Hyung Kim , Kyeong-Jun Lee
Aquaculture ( IF 4.5 ) Pub Date : 2020-07-01 , DOI: 10.1016/j.aquaculture.2020.735284 Jee Eun Han , Seong-Kyoon Choi , Se-Hyeon Han , Seung Chan Lee , Hye Jin Jeon , Chorong Lee , Kyeong Yeon Kim , Young Seo Lee , Seul Chan Park , Gahngyoon Rhee , Seon Young Park , Jun-Seob Kim , Song Park , Ji Hyung Kim , Kyeong-Jun Lee
Abstract This report describes the detailed microbial and histological characteristics of Vibrio parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (VpAHPND) that infected Pacific white shrimp (Penaeus vannamei) cultured in Korea. Shrimps showing typical symptoms of AHPND, including a whitish hepatopancreas and empty gut, were reported at two private shrimp farms in Korea in 2019. In the hepatopancreas of the field samples, sloughing of undifferentiated cells in the hepatopancreatic tubule epithelium was observed upon histopathological examination, and 2 Vibrio spp.-related toxin genes (pirA and pirB) associated with AHPND were detected in the samples. Among samples obtained from the hepatopancreas, pond water, and feces (pond sediment), the copy number of the virulent AHPND plasmid was the highest in the hepatopancreas samples, followed by that in the pond water and feces (pond sediment) samples. Two VpAHPND strains were isolated from pond water and diseased shrimp. The genome of the virulent AHPND plasmid pVp_Kor-D1–2 (GenBank accession no. CP046414.1 ), isolated from strain 19–021-D1, was fully sequenced. We confirmed that the Korean VpAHPND strain harbored the Asian-type AHPND plasmid, which was almost identical to the AHPND-associated V. owensii plasmid pVOWZ2 previously identified in China. Furthermore, the pathogenicity of the Korean VpAHPND strains was evaluated in laboratory bioassays, and we verified that those isolates were highly pathogenic to Pacific white shrimp, causing high cumulative mortality within 3 days.
更新日期：2020-07-01
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