Conventional lateral flow test strip (LFTS) sensors are insufficiently accurate and reliable due to their single-target detection with limited sample information in a single test. The increasing demand for the simultaneous determination of multiple analytes has recently been accelerating the rapid development of high-throughput and multiplexed LFTS sensing technologies. In this contribution, we systematically summarize the recent achievements on the design, development, and application of multiplexed LFTS sensors for improved rapid on-site diagnostics. The discussion focuses on emerging design strategies to increase multiplexing capacity for enhancing analytical efficiency and precision. As a proof-of-concept, several typical examples are presented. The advantages and disadvantages of such approaches are critically analyzed. Finally, we briefly discuss the current challenges and future perspectives.

常规的侧向流动试纸（LFTS）传感器由于单目标检测且一次测试中的样本信息有限，因此准确性和可靠性不足。对于同时测定多种分析物的需求不断增长，最近加速了高通量和多路LFTS传感技术的快速发展。在此贡献中，我们系统地总结了在多路LFTS传感器的设计，开发和应用方面的最新成就，以改进快速的现场诊断。讨论的重点是新兴的设计策略，以提高多路复用能力以提高分析效率和精度。作为概念验证，提供了几个典型示例。严格分析了这种方法的优缺点。最后，