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Comprehensive analysis of indels in whole-genome microsatellite regions and microsatellite instability across 21 cancer types
Genome Research ( IF 6.2 ) Pub Date : 2020-03-01 , DOI: 10.1101/gr.255026.119
Akihiro Fujimoto 1, 2, 3 , Masashi Fujita 1 , Takanori Hasegawa 4 , Jing Hao Wong 2, 3 , Kazuhiro Maejima 1 , Aya Oku-Sasaki 1 , Kaoru Nakano 1 , Yuichi Shiraishi 5, 6 , Satoru Miyano 4, 6 , Go Yamamoto 7 , Kiwamu Akagi 7 , Seiya Imoto 4 , Hidewaki Nakagawa 1
Affiliation  

Microsatellites are repeats of 1- to 6-bp units, and approximately 10 million microsatellites have been identified across the human genome. Microsatellites are vulnerable to DNA mismatch errors and have thus been used to detect cancers with mismatch repair deficiency. To reveal the mutational landscape of microsatellite repeat regions at the genome level, we analyzed approximately 20.1 billion microsatellites in 2717 whole genomes of pan-cancer samples across 21 tissue types. First, we developed a new insertion and deletion caller (MIMcall) that takes into consideration the error patterns of different types of microsatellites. Among the 2717 pan-cancer samples, our analysis identified 31 samples, including colorectal, uterus, and stomach cancers, with a higher proportion of mutated microsatellite (≥0.03), which we defined as microsatellite instability (MSI) cancers of genome-wide level. Next, we found 20 highly mutated microsatellites that can be used to detect MSI cancers with high sensitivity. Third, we found that replication timing and DNA shape were significantly associated with mutation rates of microsatellites. Last, analysis of mutations in mismatch repair genes showed that somatic SNVs and short indels had larger functional impacts than germline mutations and structural variations. Our analysis provides a comprehensive picture of mutations in the microsatellite regions and reveals possible causes of mutations, as well as provides a useful marker set for MSI detection.

中文翻译:


全面分析 21 种癌症类型的全基因组微卫星区域的插入缺失和微卫星不稳定性



微卫星是 1 至 6 bp 单位的重复序列,人类基因组中已鉴定出大约 1000 万个微卫星。微卫星很容易受到 DNA 错配错误的影响,因此已被用来检测具有错配修复缺陷的癌症。为了揭示基因组水平上微卫星重复区域的突变情况,我们分析了 21 种组织类型的泛癌样本的 2717 个全基因组中的约 201 亿个微卫星。首先,我们开发了一种新的插入和删除调用程序(MIMcall),它考虑了不同类型微卫星的错误模式。在2717个泛癌样本中,我们的分析发现了31个样本,包括结直肠癌、子宫癌和胃癌,其中微卫星突变比例较高(≥0.03),我们将其定义为全基因组水平的微卫星不稳定性(MSI)癌症。接下来,我们发现了20个高度突变的微卫星,可用于高灵敏度检测MSI癌症。第三,我们发现复制时间和 DNA 形状与微卫星的突变率显着相关。最后,对错配修复基因突变的分析表明,体细胞 SNV 和短插入缺失比种系突变和结构变异具有更大的功能影响。我们的分析提供了微卫星区域突变的全面图片,揭示了突变的可能原因,并为 MSI 检测提供了有用的标记集。
更新日期:2020-03-01
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