In this study, to improve the efficiency of an aqueous two-phase system (ATPS), a different scenario has been proposed. To this purpose, an endeavor has been undertaken to find a nanoparticle suitable for designing a process of favorable economic separation as well as to unravel the mechanism of the partitioning of nisin along with nanoparticle. First, the capabilities of several different nanoparticles were assessed. These nanoparticles were regarded as additives apt to improve nisin partitioning in the ATPS containing polyethylene glycol (with different molecular weights) and a mixture of potassium hydrogen phosphate and potassium dihydrogen phosphate. The impact of the addition of the modified nanographite (in very small amounts) on the improvement of the nisin partition coefficient in polymer/salt ATPS was quite remarkable. The results showed that the partition coefficient of nisin was increased up to 610% (eight times more than that of the ATPS without nanoparticles) in PEG 10 000 + KH₂PO₄/K₂HPO₄ + H₂O. The mechanism of the partitioning of nisin along with nanoparticle dominating the boost in the partition coefficient was explained by the structural investigations.

中文翻译：

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两相水相体系分离乳链菌肽的新工艺：具有纳米粒子的新方法

在这项研究中，为提高水相两相系统（ATPS）的效率，提出了另一种方案。为此目的，已经进行了努力以找到适合设计有利于经济分离的方法以及揭示乳链菌肽与纳米颗粒一起分配的机理的纳米颗粒。首先，评估了几种不同纳米颗粒的能力。这些纳米颗粒被认为是易于改善乳酸链球菌素在含聚乙二醇（具有不同分子量）以及磷酸氢二钾和磷酸二氢钾的混合物的ATPS中分配的添加剂。添加改性纳米石墨（非常少量）对改善聚合物/盐ATPS中乳链菌肽分配系数的影响非常显着。₂ PO ₄ / K ₂ HPO ₄ + H ₂ O.乳链菌肽与纳米颗粒占据在分配系数升压沿着分隔的机理是由结构调查说明。