当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Mosaic Immunoassays Integrated with Microfluidic Channels for High-Throughput Parallel Detection.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-04-03 , DOI: 10.1021/acs.analchem.0c00537 Fengyi Zheng 1 , Enqi He 1 , Zhongyan Wang 1 , Jiasheng Huang 1 , Zhihong Li 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-04-03 , DOI: 10.1021/acs.analchem.0c00537 Fengyi Zheng 1 , Enqi He 1 , Zhongyan Wang 1 , Jiasheng Huang 1 , Zhihong Li 1
Affiliation
|
Using the ice-printing technique, we have integrated micromosaic immunoassays (μMIAs) with microfluidic channels, which reduces the sample consumption and response time and allows high-throughput parallel detection. The ice-printing method is a low-temperature and contaminant-free process, which is more convenient, precise, and biofriendly than the traditional fabrication method. Meanwhile, based on the ice-drying process, this method can obtain a uniform distribution of the residue protein patterns, which leads to a uniform fluorescence result. As a proof of concept, the test of stability, sensitivity, and specificity of μMIA based on one-step ELISA are demonstrated. In this device, immobilized antigens surrounded with ice could remain biological at -20 °C for months.
中文翻译：
镶嵌免疫分析与微流控通道集成，可进行高通量并行检测。
使用冰印技术，我们将微马赛克免疫分析（μMIA）与微流体通道集成在一起，从而减少了样品消耗和响应时间，并允许高通量并行检测。冰印法是一种低温，无污染的工艺，比传统的制造方法更加方便，精确和生物友好。同时，在冰干过程的基础上，该方法可以获得残留蛋白质图案的均匀分布，从而导致均匀的荧光结果。作为概念验证，证明了基于一步ELISA的μMIA的稳定性，敏感性和特异性测试。在这种装置中，被冰包围的固定抗原可以在-20°C下保持数月的生物学状态。
更新日期：2020-04-23
中文翻译：
镶嵌免疫分析与微流控通道集成，可进行高通量并行检测。
使用冰印技术，我们将微马赛克免疫分析（μMIA）与微流体通道集成在一起，从而减少了样品消耗和响应时间，并允许高通量并行检测。冰印法是一种低温，无污染的工艺，比传统的制造方法更加方便，精确和生物友好。同时，在冰干过程的基础上，该方法可以获得残留蛋白质图案的均匀分布，从而导致均匀的荧光结果。作为概念验证，证明了基于一步ELISA的μMIA的稳定性，敏感性和特异性测试。在这种装置中，被冰包围的固定抗原可以在-20°C下保持数月的生物学状态。
京公网安备 11010802027423号