Polypeptide-Templated Au Nanoclusters with Red and Blue Fluorescence Emissions for Multimodal Imaging of Cell Nuclei,ACS Applied Bio Materials

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Polypeptide-Templated Au Nanoclusters with Red and Blue Fluorescence Emissions for Multimodal Imaging of Cell Nuclei
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-25 , DOI: 10.1021/acsabm.9b01078
Chang Liu ₁ , Xiangchun Zhang ₂ , Xu Han ₃ , Yide Fang ₄ , Xuemei Liu ₁ , Xiaolin Wang ₁ , Geoffrey I N Waterhouse _{1,

5} , Chao Xu ₁ , Hongzong Yin ₁ , Xueyun Gao ₆

Affiliation

Noble metal nanoclusters (<2 nm) prepared using special molecules such as proteins and peptides as templates have been attracting increasing attention in biomedical applications and cell imaging because of their excellent biocompatibility, size-dependent fluorescence properties, and relatively simple synthesis. Although cell imaging using metal clusters has developed rapidly in recent years, multimodal imaging of the cell nucleus remains challenging. Herein, we report the successful synthesis of two different types of gold nanoclusters, one with blue fluorescence emission (λ_ex 320 nm, λ_em 405 nm) and the other with red fluorescence emission (λ_ex 560 nm, λ_em 657 nm), using the same polypeptide SV (sequence: NH₂–CCYGGPKKKRKVG-COOH) as a template. The synthesis of these two optically distinct Au nanoclusters (Au NCs) was achieved through the optimization of synthesis conditions, including light, pH, the Au: polypeptide ratio, reaction time, and temperature. The multimodal imaging ability of the Au NCs were subsequently explored, with particular emphasis on the enzyme-like catalysis of the NCs and their use in the optical imaging of cell nuclei. Results show that the Au NCs possessed similar biological activities as the parent peptide used in their synthesis as well as demonstrating peroxidase-like activity due to the Au nanocluster core. Further, the Au NCs targeted the cell nucleus, with their strong fluorescence allowing cell imaging (cells containing Au clusters could be distinguished by eye, eliminating the need for additional labels). Finally, the Au NCs exhibited excellent multimodal cell imaging abilities. The results suggest that the Au NCs introduced here are promising candidates for the labeling and analysis of pathological samples.

中文翻译：

具有红色和蓝色荧光发射的多肽模板金纳米簇用于细胞核的多模式成像

以蛋白质和肽等特殊分子为模板制备的贵金属纳米团簇（<2 nm）由于其优异的生物相容性、尺寸依赖性荧光特性和相对简单的合成，在生物医学应用和细胞成像中引起了越来越多的关注。尽管使用金属簇的细胞成像近年来发展迅速，但细胞核的多模态成像仍然具有挑战性。在这里，我们报告了两种不同类型的金纳米团簇的成功合成，一种具有蓝色荧光发射（λ _ex 320 nm，λ _em 405 nm），另一种具有红色荧光发射（λ _ex 560 nm，λ _em 657 nm），使用相同的多肽 SV（序列：NH ₂–CCYGGPKKKRKVG-COOH）作为模板。这两种光学上不同的金纳米团簇（Au NCs）的合成是通过优化合成条件来实现的，包括光、pH、Au：多肽比、反应时间和温度。随后探索了 Au NCs 的多模态成像能力，特别强调了 NCs 的酶样催化及其在细胞核光学成像中的应用。结果表明，Au NCs 具有与其合成中使用的母肽相似的生物活性，并且由于 Au 纳米簇核心而表现出类似过氧化物酶的活性。此外，Au NCs 靶向细胞核，其强荧光允许细胞成像（含有 Au 簇的细胞可以通过肉眼区分，无需额外的标记）。最后，Au NCs 表现出优异的多模式细胞成像能力。结果表明，这里介绍的 Au NC 是病理样本标记和分析的有希望的候选者。

更新日期：2020-04-23

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