Studies on bacterial physiology are incomplete without knowledge of the signalling and regulatory systems that a bacterium uses to sense and respond to its environment. Two‐component systems (TCSs) are among the most prevalent bacterial signalling systems, and they control essential and secondary physiological processes; however, even in model organisms, we lack a complete understanding of the signals sensed, the phosphotransfer partners and the functions regulated by these systems. In this review, we discuss several tools to map the genes targeted by transcriptionally acting TCSs. Many of these tools have been used for studying individual TCSs across diverse species, but systematic approaches to delineate entire signalling networks have been very few. Since genome sequences and high‐throughput technologies are now readily available, the methods presented here can be applied to characterize the entire DNA‐binding TCS signalling network in any bacterial species and are especially useful for non‐model environmental bacteria.

中文翻译：

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定位细菌两成分系统响应调节剂靶基因的工具。

如果不了解细菌用来感知和响应其环境的信号传导和调控系统，那么细菌生理学的研究是不完整的。两成分系统（TCS）是最流行的细菌信号系统之一，它们控制基本和次要生理过程。但是，即使在模型生物中，我们也对所感测的信号，磷酸转移伴侣和这些系统调节的功能缺乏全面的了解。在这篇综述中，我们讨论了几种工具来定位转录作用的TCSs靶向的基因。这些工具中的许多已用于研究跨物种的单个TCS，但是描述整个信号网络的系统方法很少。由于基因组序列和高通量技术现在很容易获得，