DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables,Microbial Biotechnology

当前位置： X-MOL 学术 › Microb. Biotechnol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-03-24 , DOI: 10.1111/1751-7915.13560
Youming Shen ₁ , Jiyun Nie _{1,

2} , Lixue Kuang ₁ , Jianyi Zhang ₁ , Haifei Li ₁

Affiliation

The development of DNA sequencing technology has provided an effective method for studying foodborne and phytopathogenic microorganisms on fruits and vegetables (F & V). DNA sequencing has successfully proceeded through three generations, including the tens of operating platforms. These advances have significantly promoted microbial whole‐genome sequencing (WGS) and DNA polymorphism research. Based on genomic and regional polymorphisms, genetic markers have been widely obtained. These molecular markers are used as targets for PCR or chip analyses to detect microbes at the genetic level. Furthermore, metagenomic analyses conducted by sequencing the hypervariable regions of ribosomal DNA (rDNA) have revealed comprehensive microbial communities in various studies on F & V. This review highlights the basic principles of three generations of DNA sequencing, and summarizes the WGS studies of and available DNA markers for major bacterial foodborne pathogens and phytopathogenic fungi found on F & V. In addition, rDNA sequencing‐based bacterial and fungal metagenomics are summarized under three topics. These findings deepen the understanding of DNA sequencing and its application in studies of foodborne and phytopathogenic microbes and shed light on strategies for the monitoring of F & V microbes and quality control.

中文翻译：

果蔬中微生物的DNA测序，基因组和遗传标记

DNA测序技术的发展为研究水果和蔬菜上的食源性和植物致病性微生物（F＆V）提供了有效的方法。DNA测序已成功进行了三代，包括数十个操作平台。这些进展极大地促进了微生物全基因组测序（WGS）和DNA多态性研究。基于基因组和区域多态性，已经广泛获得了遗传标记。这些分子标记用作PCR或芯片分析的靶标，以检测遗传水平的微生物。此外，通过对核糖体DNA（rDNA）高变区进行测序而进行的宏基因组学分析显示，在有关F＆V的各种研究中，微生物群落全面。这篇综述重点介绍了三代DNA测序的基本原理，并总结了F＆V上发现的主要细菌性食源性病原体和植物致病真菌的WGS研究以及可用的DNA标记。此外，还总结了基于rDNA测序的细菌和真菌宏基因组学。在三个主题下。这些发现加深了对DNA测序及其在食源性和植物致病性微生物研究中的应用的了解，并阐明了监测F＆V微生物和质量控制的策略。

更新日期：2020-03-24

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11