An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) and indirect enzyme‐linked immunosorbent assay (ELISA) and to molecular test by polymerase chain reaction (PCR). In addition, a Phlebotomine sandflies survey was performed in the same shelters. The analyses revealed a positivity of 31.91% (30/94) through ELISA and 29.79% (28/94) through IFAT. The two serological tests showed a positive association with perfect agreement (k = 0.925). None of the cats were positive by Leishmania spp. DNA. One Lutzomyia (Lutzomyia) longipalpis male was found in one of the cat shelters. The results and the implications of our findings are discussed below.

中文翻译：

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猫收容所中的利什曼病：血清学、分子和昆虫学研究

流行病学利什曼原虫属。在巴西利什曼病流行区的猫收容所进行了昆虫学白蛉调查。从两个动物保护收容所的 94 只猫身上采集了血液和结膜拭子 (CS) 样本。使用间接免疫荧光抗体测试（IFAT）和间接酶联免疫吸附测定（ELISA）对这些样本进行血清学测试，并通过聚合酶链反应（PCR）进行分子测试。此外，还在同一避难所进行了白蛉调查。分析显示，ELISA 的阳性率为 31.91% (30/94)，IFAT 的阳性率为 29.79% (28/94)。两项血清学检测显示出正相关且完全一致 ( k  = 0.925)。这些猫均未呈利什曼原虫阳性。脱氧核糖核酸。在其中一个猫收容所中发现了一只雄性长触须蝇（Lutzomyia） 。我们的研究结果和意义将在下面讨论。