This study evaluated the effects of dietary γ‐aminobutyric acid (GABA) on the growth performance, serum biochemical indices and antioxidant status of pharaoh cuttlefish, Sepia pharaonis. Cuttlefish were cultured in open‐culturing cement pool systems for 8 weeks. Six practical diets supplemented with graded levels of GABA (0, 20, 40, 60, 80 and 100 mg/kg) were formulated. Each diet was randomly assigned to triplicate groups of 60 cuttlefish (mean weight: 10.33 g), the cuttlefish were fed two times per day to apparent satiation. The results showed that the specific growth rate (SGR), weight gain (WG) and feed efficiency (FE) significantly increased with dietary GABA supplementation (p < .05). The survival rate (SR) and protein content in muscle significantly increased when 58.9 mg/kg GABA supplied. Moreover, the nitric oxide (NO) content and acid phosphatase (ACP) activity in serum were significantly increased with dietary GABA supplementation (p < .05), while the activity of aspartate aminotransferase (AST) in serum decreased significantly when supplied with GABA at 58.9 mg/kg (p < .05). In addition, dietary GABA improved antioxidation activity by significantly increasing the activities of superoxide dismutase (SOD) and catalase (CAT) but decreasing malondialdehyde (MDA) levels in the liver and gill (p < .05). On the basis of the quadratic regression analysis of FE, the optimum content of dietary GABA in S. pharaonis was estimated to be 55.3 mg/kg. The findings of this study demonstrated that dietary GABA had a positive effect on the growth performance, serum biochemical indices and antioxidant status of S. pharaonis.

中文翻译：

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补充γ-氨基丁酸对法老墨鱼生长性能，血清生化指标和抗氧化状态的影响

本研究评估膳食γ氨基丁酸（GABA）的生长性能，血液生化指标和虎斑乌贼的抗氧化状态的影响，棕褐色蚁。乌贼在开放式水泥池系统中养殖了8周。配制了六种补充了GABA分级水平（0、20、40、60、80和100 mg / kg）的实用饮食。每种饮食被随机分为三组，每组60条墨鱼（平均体重：10.33克），每天给墨鱼喂两次两次，以使其明显饱食。结果表明，日粮中添加GABA可使比生长速率（SGR），增重（WG）和饲料效率（FE）显着增加（p < .05）。当提供58.9 mg / kg GABA时，肌肉的存活率（SR）和蛋白质含量显着增加。此外，日粮中添加GABA可使血清中的一氧化氮（NO）含量和酸性磷酸酶（ACP）活性显着增加（p <  .05），而当与GABA配合使用时，血清中的天冬氨酸转氨酶（AST）活性则显着下降。 58.9 mg / kg（p <  .05）。此外，饮食中的GABA可通过显着增加肝脏和g中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性而降低丙二醛（MDA）的水平来提高抗氧化活性（p <  .05）。在FE的二次回归分析的基础上，确定了S中日粮GABA的最佳含量。 法老王估计为55.3 mg / kg。这项研究的结果表明，饮食中的GABA对法老葡萄球菌的生长性能，血清生化指标和抗氧化状态具有积极的影响。