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Efficient cellular uptake of click nucleic acid modified proteins
Chemical Communications ( IF 6.164 ) Pub Date : 2020/03/25 , DOI: 10.1039/c9cc09401f
Albert Harguindey; Heidi R. Culver; Jasmine Sinha; Christopher N. Bowman; Jennifer N. Cha

Efficient intracellular delivery of biomacromolecules such as proteins continues to remain a challenge despite its potential for medicine. In this work, we show that mScarlet, a non cytotoxic red fluorescent protein (RFP) conjugated to Click Nucleic Acid (CNA), a synthetic analog of DNA, undergo cell uptake significantly more than either native proteins or proteins conjugated with similar amounts of DNA in MDA-MB-468 cells. We further demonstrate that the process of cell uptake is metabolically driven and that scavenger receptors and caveolae mediated endocytosis play a significant role. Co-localization studies using anti-scavenger receptor antibodies suggest that scavenger receptors are implicated in the mechanism of uptake of CNA modified proteins.
更新日期:2020-04-01

 

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