Reliable and sensitive detection of Staphylococcal enterotoxin B (SEB) is important to prevent potential human health risk from foods contaminated by SEB. Here, a triple-channel assay was developed for ultrasensitive detection of SEB. Based on the optical characteristics of tetramers composed of gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs), the SEB amounts in food products can be simultaneously detected by using surface-enhanced Raman scattering (SERS), fluorometry, and circular dichroism (CD). Under SERS mode, fluorescence mode, and CD mode, wide linear detection ranges (1–750 pg/mL, 1–750 pg/mL, and 2–500 pg·mL⁻¹) were achieved. Furthermore, the practicality of the novel assay was evaluated by determining SEB contents in spiked milk samples. These results indicate that the triple-mode aptasensor is a potential useful tool in quantitative detection of SEB in foods.

可靠，灵敏地检测葡萄球菌肠毒素B（SEB）对于防止受到SEB污染的食物对人类健康的潜在危害非常重要。在这里，开发了一种三通道测定法，用于SEB的超灵敏检测。根据由金纳米颗粒（AuNPs）和上转换纳米颗粒（UCNPs）组成的四聚体的光学特性，可以通过使用表面增强拉曼散射（SERS），荧光法和圆二色性（CD）同时检测食品中的SEB量。 。在SERS模式，荧光模式和CD模式下，线性检测范围宽（1–750 pg / mL，1–750 pg / mL和2–500 pg·mL ^-1）达成。此外，通过测定加标牛奶样品中的SEB含量评估了该新型测定的实用性。这些结果表明，三模式适体传感器是定量检测食品中SEB的潜在有用工具。