In this study, non-animal mushroom carboxymethyl chitosan (NAM-CMCS) was used as a natural polymer stabilizing agent in the ultrasonic preparation of a ZnO nanocomposite at ambient laboratory temperatures. The formation and morphology of the ZnO nanoparticles were investigated by applying FTIR, XRD, XPS, FE-SEM, and DLS techniques. The FTIR and XPS spectra confirmed the presence of NAM-CMCS functional groups and ZnO in the nanoparticles. The prepared NAM-CMCS-ZnO nanoparticles were shown by FE-SEM to have a spherical shape and an average diameter of 18 ± 3.6 nm. The DLS-determined size distribution showed the NAM-CMCS-ZnO nanoparticle size averaged 21 ± 2.9 nm. Finally, cytocompatibility, hemostasis, and antibacterial performance were assessed in vitro to evaluate the biological performance of NAM-CMCS-ZnO nanoparticles. In vitro Prestoblue® assay and live/dead test results from skin fibroblast and keratinocytes confirmed the developed NAM-CMCS-ZnO nanoparticles were biocompatible over a wide range of concentrations (0-500 μg/well). The NAM-CMCS-ZnO nanoparticles exhibited synergetic antibacterial properties against Gram-positive (Staphylococcus aureus) bacteria. Moreover, the nanoparticles showed hemostatic properties with good hemocompatibility. The overall excellent biological properties of NAM-CMCS-ZnO nanoparticles indicate its suitability for use in wound dressing applications.

中文翻译：

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止血，生物相容性和抗菌的非动物性真菌蘑菇基羧甲基壳聚糖-ZnO纳米复合材料，可用于伤口愈合。

在这项研究中，非动物性蘑菇羧甲基壳聚糖（NAM-CMCS）被用作在实验室环境温度下超声制备ZnO纳米复合材料的天然聚合物稳定剂。利用FTIR，XRD，XPS，FE-SEM和DLS技术研究了ZnO纳米粒子的形成和形貌。FTIR和XPS光谱证实了纳米颗粒中存在NAM-CMCS官能团和ZnO。所制备的NAM-CMCS-ZnO纳米颗粒通过FE-SEM显示为球形，平均直径为18±3.6nm。DLS确定的尺寸分布显示NAM-CMCS-ZnO纳米颗粒的平均尺寸为21±2.9 nm。最后，在体外评估细胞相容性，止血和抗菌性能，以评估NAM-CMCS-ZnO纳米颗粒的生物学性能。皮肤成纤维细胞和角质形成细胞的体外Prestoblue®分析和活/死测试结果证实，开发的NAM-CMCS-ZnO纳米颗粒在广泛的浓度范围内（0-500μg/孔）具有生物相容性。NAM-CMCS-ZnO纳米颗粒对革兰氏阳性（金黄色葡萄球菌）细菌表现出协同抗菌性能。此外，纳米颗粒显示出具有良好的血液相容性的止血性质。NAM-CMCS-ZnO纳米颗粒的总体优异生物学特性表明其适用于伤口包扎应用。NAM-CMCS-ZnO纳米颗粒对革兰氏阳性（金黄色葡萄球菌）细菌表现出协同抗菌性能。此外，纳米颗粒显示出具有良好的血液相容性的止血性质。NAM-CMCS-ZnO纳米颗粒的总体优异生物学特性表明其适用于伤口包扎应用。NAM-CMCS-ZnO纳米颗粒对革兰氏阳性（金黄色葡萄球菌）细菌具有协同抗菌性能。此外，纳米颗粒显示出具有良好的血液相容性的止血性质。NAM-CMCS-ZnO纳米颗粒的总体优异生物学特性表明其适用于伤口包扎应用。