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Influence of light and temperature cycles on the expression of circadian clock genes in the mussel Mytilus edulis.
Marine Environmental Research ( IF 3.0 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.marenvres.2020.104960 Emma C Chapman 1 , Brodie J Bonsor 2 , Daniel R Parsons 3 , Jeanette M Rotchell 1
Marine Environmental Research ( IF 3.0 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.marenvres.2020.104960 Emma C Chapman 1 , Brodie J Bonsor 2 , Daniel R Parsons 3 , Jeanette M Rotchell 1
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Clock genes and environmental cues regulate essential biological rhythms. The blue mussel, Mytilus edulis, is an ecologically and economically important intertidal bivalve undergoing seasonal reproductive rhythms. We previously identified seasonal expression differences in M. edulis clock genes. Herein, the effects of light/dark cycles, constant darkness, and daily temperature cycles on the circadian expression patterns of such genes are characterised. Clock genes Clk, Cry1, ROR/HR3, Per and Rev-erb/NR1D1, and Timeout-like, show significant mRNA expression variation, persisting in darkness indicating endogenous control. Rhythmic expression was apparent under diurnal temperature cycles in darkness for all except Rev-erb. Temperature cycles induced a significant expression difference in the non-circadian clock-associated gene aaNAT. Furthermore, Suppression Subtractive Hybridisation (SSH) was used to identify seasonal genes with potential links to molecular clock function and revealed numerous genes meriting further investigation. Understanding the relationship between environmental cues and molecular clocks is crucial in predicting the outcomes of environmental change on fundamental rhythmic processes.
中文翻译：
光和温度循环对贻贝贻贝中昼夜节律基因表达的影响。
时钟基因和环境提示调节基本的生物节律。蓝贻贝（Mytilus edulis）是一种具有重要生态和经济意义的潮间双壳类，经历季节性的生殖节律。我们以前确定了可食的支原体时钟基因的季节性表达差异。在此，表征了亮/暗周期，恒定的黑暗和每日温度周期对此类基因的昼夜表达模式的影响。时钟基因Clk，Cry1，ROR / HR3，Per和Rev-erb / NR1D1和Timeout-like表现出显着的mRNA表达变化，在黑暗中持续存在，表明有内源性控制。除Rev-erb外，其余所有动物在昼夜温度循环下的节律性表达都很明显。温度周期诱导非昼夜节律相关基因aaNAT的显着表达差异。此外，抑制消减杂交（SSH）被用于鉴定与分子时钟功能有潜在联系的季节性基因，并揭示了许多值得进一步研究的基因。了解环境线索与分子钟之间的关系对于预测基本节律过程中环境变化的结果至关重要。
更新日期：2020-03-26
中文翻译：
光和温度循环对贻贝贻贝中昼夜节律基因表达的影响。
时钟基因和环境提示调节基本的生物节律。蓝贻贝（Mytilus edulis）是一种具有重要生态和经济意义的潮间双壳类，经历季节性的生殖节律。我们以前确定了可食的支原体时钟基因的季节性表达差异。在此，表征了亮/暗周期，恒定的黑暗和每日温度周期对此类基因的昼夜表达模式的影响。时钟基因Clk，Cry1，ROR / HR3，Per和Rev-erb / NR1D1和Timeout-like表现出显着的mRNA表达变化，在黑暗中持续存在，表明有内源性控制。除Rev-erb外，其余所有动物在昼夜温度循环下的节律性表达都很明显。温度周期诱导非昼夜节律相关基因aaNAT的显着表达差异。此外，抑制消减杂交（SSH）被用于鉴定与分子时钟功能有潜在联系的季节性基因，并揭示了许多值得进一步研究的基因。了解环境线索与分子钟之间的关系对于预测基本节律过程中环境变化的结果至关重要。
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