Construction of hybrid DNAs@CP for the rapid synchronous sensing of multiplex microRNAs based on experimental studies and molecular simulation.,Journal of Inorganic Biochemistry

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Construction of hybrid DNAs@CP for the rapid synchronous sensing of multiplex microRNAs based on experimental studies and molecular simulation.
Journal of Inorganic Biochemistry ( IF 3.8 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.jinorgbio.2020.111076
Zi-Chuan Yang ₁ , Tao-Rui Wang ₂ , Rong-Tian Li ₁ , Ting-Wei Chen ₁ , Yue Fan ₁ , Wei-Hua Jiang ₁ , Min-Min Li ₂ , Jin-Xiang Chen ₁

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A water stable one-dimensional (1D) ladder-shaped coordination polymer (CP) has been synthesized and exhibits a strong affinity to two fluorescein-tagged single-stranded probe DNAs (P-DNAs), giving a sensing platform of P-DNAs@1. Such a hybrid sensing platform is capable of simultaneous detection of breast cancer related microRNA-221 (miRNA-221) and miRNA-222 in a specific and synchronous manner, without observable cross-reactions, as supported by experimental evidences. The interaction mode and the electronic energy between CP 1 with nucleic acid were confirmed by molecular simulation and the universal force field (UFF).

中文翻译：

基于实验研究和分子模拟，构建用于快速同步检测多重microRNA的杂化DNAs @ CP。

合成了一种水稳定的一维（1D）梯形配位聚合物（CP），它对两个荧光标记的单链探针DNA（P-DNA）表现出很强的亲和力，为P-DNAs提供了传感平台1。这样的混合感测平台能够以特异性和同步方式同时检测与乳腺癌相关的microRNA-221（miRNA-221）和miRNA-222，而没有可观察到的交叉反应，实验证据支持。通过分子模拟和万向力场（UFF）证实了CP 1与核酸之间的相互作用方式和电能。

更新日期：2020-03-23

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