Abstract Amino-rich thin coatings, known to show superior cell adhesion, proliferation and viability, are prepared in this study by means of plasma polymerisation. The Yasuda parameter (W/FM) is used as a scaling factor for the deposition of cyclopropylamine based plasma polymer films (CPA-PPFs) making use of a dielectric barrier discharge (DBD) operated at sub-atmospheric pressure. In this paper, the experimental research is focussed on the stability of the CPA-PPFs in water, a property of PPFs which is often overlooked in literature. More specifically, after their plasma-assisted fabrication, the polymerised samples are immersed in water for 24 h after which the coatings are chemically evaluated using Fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy. The obtained results reveal that for all W/FM values under study water immersion results in a significant loss in C N/C N groups and a shift of primary to secondary/tertiary amines combined with the incorporation of oxygen at the surface as amides and alcohols/ethers. Moreover, FTIR, XPS chemical depth profiling (by means of C60 sputtering) and scanning electron microscopy (SEM) results also reveal that at W/FM values ≤ 288 MJ/kg, the PPFs are gradually dissolving during water immersion, while at W/FM values > 288 MJ/kg, the PPFs are delaminating from the substrate due to their high crosslinking degree. Nevertheless, cell-coating interaction studies reveal no signs of cytotoxicity 1 day after cell seeding and show that the thin coatings remaining on the substrate still strongly increase the adhesion of fibroblasts. In the near future, efforts will be undertaken to avoid the delamination from the substrate of the PPFs prepared at high W/FM values.

中文翻译：

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研究基于环丙胺的等离子体聚合物在水中的稳定性

摘要 本研究通过等离子体聚合制备了富含氨基的薄涂层，已知显示出优异的细胞粘附、增殖和活力。Yasuda 参数 (W/FM) 用作使用在亚大气压下操作的介质阻挡放电 (DBD) 沉积环丙胺基等离子体聚合物薄膜 (CPA-PPF) 的比例因子。在本文中，实验研究的重点是 CPA-PPFs 在水中的稳定性，这是 PPFs 在文献中经常被忽视的特性。更具体地说，在等离子体辅助制造后，将聚合样品浸入水中 24 小时，然后使用傅里叶变换红外 (FTIR) 和 X 射线光电子 (XPS) 光谱对涂层进行化学评估。获得的结果表明，对于研究中的所有 W/FM 值，水浸导致 CN/CN 基团的显着损失和伯胺向仲/叔胺的转变，以及在表面以酰胺和醇/醚形式掺入的氧. 此外，FTIR、XPS 化学深度分析（通过 C60 溅射）和扫描电子显微镜 (SEM) 结果还表明，在 W/FM 值≤ 288 MJ/kg 时，PPFs 在水浸过程中逐渐溶解，而在 W/ FM 值 > 288 MJ/kg，PPF 由于其高交联度而与基材分层。然而，细胞涂层相互作用研究显示细胞接种后 1 天没有细胞毒性迹象，并表明残留在基材上的薄涂层仍然强烈增加成纤维细胞的粘附。在不远的将来，