T-2 toxin, one of the most toxic mycotoxins, is commonly presented along with its metabolites, HT-2 toxin, neosolaniol (NEO), T-2 triol, and T-2 tetraol in foodstuff and feed. The aim of this study was to evaluate the cytotoxic effects of T-2 toxin alone and in combination with its metabolites on porcine Leydig cells. Based on the determination of cell viability with CCK-8, toxicological interactions were investigated using Combination Index method. The cytotoxic potency of five tested mycotoxins individual and their mixtures all showed with a dose-dependent manner. In view of IC50 values, the decreasing cytotoxicity of mycotoxins was ranking: T-2 toxin > HT-2 toxin > T-2 triol > NEO > T-2 tetraol. Combinations of T-2+HT-2, T-2+NEO, and HT-2+NEO displayed synergism at low doses but antagonism at high doses, while the ternary combination of T-2+HT-2+NEO revealed adverse situation from antagonism to synergism. All binary and ternary combinations of T-2 toxin, T-2 triol, and T-2 tetraol exhibited antagonistic interactions. Our results suggest that the co-occurrence of T-2 toxin and its metabolites might pose a slight threat to reproductive health due to antagonistic interactions. However, the synergy observed should be not ignored especially at low doses of mycotoxins co-occurrence in the diet.

中文翻译：

---

T-2毒素及其四种代谢产物对猪Leydig细胞的个体和联合细胞毒作用。

T-2毒素是毒性最高的霉菌毒素之一，通常与它的代谢产物，HT-2毒素，新茄尼醇（NEO），T-2三醇和T-2四醇一起存在于食品和饲料中。这项研究的目的是评估单独的T-2毒素及其代谢产物对猪Leydig细胞的细胞毒性作用。在用CCK-8测定细胞活力的基础上，使用组合指数方法研究了毒理学相互作用。五个受测真菌毒素个体及其混合物的细胞毒性效力均呈剂量依赖性。鉴于IC50值，霉菌毒素的细胞毒性下降的排名为：T-2毒素> HT-2毒素> T-2三醇> NEO> T-2四醇。T-2 + HT-2，T-2 + NEO和HT-2 + NEO的组合在低剂量时显示出协同作用，但在高剂量时显示出拮抗作用，T-2 + HT-2 + NEO的三元组合显示了从拮抗作用到协同作用的不利情况。T-2毒素，T-2三醇和T-2四醇的所有二元和三元组合均表现出拮抗作用。我们的结果表明，由于拮抗作用，T-2毒素及其代谢产物的同时存在可能对生殖健康构成轻微威胁。但是，观察到的协同作用不容忽视，尤其是在饮食中低剂量真菌毒素同时存在时。