Abstract Hydroxyapatite is a multifunctional biomaterial that combines biocompatibility and bioactivity for various biomedical applications such as bone repairing and bioimaging. In the present study nano-hydroxyapatite (n-HAp) was synthesized using microwave irradiation technique. Subsequently, the MgO was introduced into the n-HAp matrix and various bioactive compositions of HAp-MgO nanocomposites were fabricated. The structural, mechanical, in vivo cell viability, and in vivo imaging properties of these nanocomposites were studied. The XRD results show that the composites sintered at 1200 °C, n-HAp partially decomposed into beta-tricalcium phosphate (β-TCP). The sintered density of the composites varying from 2.72 ± 0.066 to 3.03 ± 0.093 g cm−3 with the addition of 0.0–2.0 wt % of MgO. As increasing the amounts of MgO, a remarkable increase in the mechanical properties of the composite was achieved. The composite HAp-1.0MgO exhibited the highest mechanical properties with a compressive strength of 111.20 ± 5 MPa, fracture toughness 136.98 ± 5 MJ/m3 and revealed much amplification than pure n-HAp. Thus, the addition of MgO acting as an excellent mechanical reinforcing agent. The surface morphology of the composites revealed a significant change in the porous surface to denser. The low contact angle revealed the considerable hydrophilic nature of the composite surface. The biological study of these nano-composites with Drosophila third instar larvae indicated comparable or more favorable biocompatibility in terms of cell viability. Also internalized by Drosophila third instar larvae exhibited fluorescence under green and red filters using epifluorescence microscopy. Thus, the fabricated HAp-MgO nanocomposites with excellent biological properties are expected to be a multifunctional bioactive material for bone tissue regeneration and cell imaging applications.

中文翻译：

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添加氧化镁的羟基磷灰石纳米复合材料的机械性能和亲水性能增强：一种用于承重应用的骨替代材料

摘要 羟基磷灰石是一种兼具生物相容性和生物活性的多功能生物材料，可用于骨修复和生物成像等多种生物医学应用。在本研究中，纳米羟基磷灰石 (n-HAp) 是使用微波辐射技术合成的。随后，将 MgO 引入 n-HAp 基质中，并制备了 HAp-MgO 纳米复合材料的各种生物活性成分。研究了这些纳米复合材料的结构、机械、体内细胞活力和体内成像特性。XRD 结果表明复合材料在 1200 °C 下烧结，n-HAp 部分分解为 β-磷酸三钙（β-TCP）。复合材料的烧结密度从 2.72 ± 0.066 变化到 3.03 ± 0.093 g cm-3，添加 0.0-2.0 wt% MgO。随着 MgO 含量的增加，实现了复合材料机械性能的显着提高。复合材料 HAp-1.0MgO 表现出最高的机械性能，抗压强度为 111.20 ± 5 MPa，断裂韧性为 136.98 ± 5 MJ/m3，并且比纯 n-HAp 具有更高的放大率。因此，添加 MgO 作为一种优异的机械增强剂。复合材料的表面形态显示多孔表面发生显着变化，变得更致密。低接触角表明复合材料表面具有相当大的亲水性。这些纳米复合材料与果蝇三龄幼虫的生物学研究表明，在细胞活力方面具有相当或更有利的生物相容性。也被果蝇三龄幼虫内化，使用落射荧光显微镜在绿色和红色滤光片下表现出荧光。因此，制备的具有优异生物学特性的 HAp-MgO 纳米复合材料有望成为用于骨组织再生和细胞成像应用的多功能生物活性材料。