Abstract In this article, NiFe2O4 nanoparticles (NPs) were prepared by co-precipitation method with subsequent thermal annealing leading to obtainment NPs with average sizes of 78 nm. Structure and magnetic analysis were performed by X-ray diffraction, transmission electron microscopy, Mossbauer spectroscopy and vibrating sample magnetometry techniques. Stability of NiFe2O4 NPs was evaluated in PBS solution during 20 days. The toxicity of prepared NPs was evaluated in vitro using different cancer cell lines: HeLa (cervical cancer cell), PC-3 (prostate cancer cell). Fibroblasts like cells of L929 obtained from subcutaneous adipose tissue of mouse were used as normal cells. Results indicate successful synthesis of NiFe2O4 NPs that exhibit low cytotoxicity in concentration range from 1 to 100 μg/ml. Presented physical and biological results indicate the possibility of application investigated magnetic nanoparticles in hyperthermia, targeted drug delivery, magnetic resonance imaging or cell separation.

中文翻译：

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共沉淀和后续热退火合成的 NiFe2O4 纳米复合材料的稳定性和细胞毒性研究

摘要 在本文中，NiFe2O4 纳米粒子 (NPs) 通过共沉淀法制备，随后的热退火导致获得平均尺寸为 78 nm 的 NPs。通过 X 射线衍射、透射电子显微镜、穆斯堡尔光谱和振动样品磁力测定技术进行结构和磁性分析。NiFe2O4 NPs 在 PBS 溶液中的稳定性在 20 天内进行了评估。使用不同的癌细胞系在体外评估了制备的 NPs 的毒性：HeLa（宫颈癌细胞）、PC-3（前列腺癌细胞）。取自小鼠皮下脂肪组织的成纤维细胞样 L929 细胞用作正常细胞。结果表明成功合成了 NiFe2O4 NPs，在 1 到 100 μg/ml 的浓度范围内表现出低细胞毒性。