Bimatoprost, latanoprost, and unoprostone are prostaglandin F2α analogs (PGAs) and are used to lower intraocular pressure. We investigated the free acid effects of these three prostaglandin analogs: bimatoprost, latanoprost, and unoprostone on human matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMP) in the trabecular meshwork (TM) cells. Immunoblot results show that all three PGAs generally increased MMPs-1,9 and TIMPs-4. Additionally, bimatoprost and latanoprost both increased MMP-3 and TIMP-2, while unoprostone had an indeterminate effect on both. Zymography results show that all three PGAs except unoprostone increased intermediate MMP-1 activity while bimatoprost and latanoprost increased MMP-9 activity. Together, these data suggest that the balance between MMPs and TIMPs correlate to the relative intraocular pressure lowering effectiveness observed in clinical studies of these PGAs.

中文翻译：

---

前列腺素类似物：拉坦前列素，比马前列素和unoprostone对人小梁网状内皮细胞基质金属蛋白酶及其抑制剂的影响。

比马前列素，拉坦前列素和unoprostone是前列腺素F2α类似物（PGA），用于降低眼内压。我们研究了这三种前列腺素类似物的游离酸作用：比马前列素，拉坦前列素和unoprostone对小梁网（TM）细胞中人基质金属蛋白酶（MMP）和金属蛋白酶组织抑制剂（TIMP）的影响。免疫印迹结果表明，所有三个PGA通常都会增加MMPs-1,9和TIMPs-4。此外，比马前列素和拉坦前列素均增加MMP-3和TIMP-2，而unoprostone对这两者均起不确定作用。谱线仪结果表明，除unoprostone以外的所有三个PGA均增加了中间MMP-1活性，而比马前列素和拉坦前列素增加了MMP-9活性。一起，