AIM The present research work aimed to prepare and characterize nanoparticles of curcumin using polymers from different sources like natural, synthetic and semi-synthetic, and compare their activity for wound healing. Curcumin, BCS class II drug, is a polyphenol with proven wound healing activity. MATERIAL AND METHODS The curcumin-loaded chitosan and carboxymethylcellulose (CMC) nanoparticles were prepared by ionotropic gelation method. In contrast, poly-lactic co-glycolic acid (PLGA) nanoparticles were prepared by a double emulsion solvent evapouration method. The different sources of polymers include natural (chitosan), synthetic (PLGA) and semi-synthetic CMC were used for the preparation of nanoparticles. KEY FINDINGS The percentage entrapment efficiency of curcumin-loaded nanoparticles was found to be in the order of polymers PLGA>chitosan>CMC. The in-vitro release study of carboxymethyl cellulose -curcumin nanoparticles was found to be 74.96% for 24 h. The presence of a specific peak of curcumin in all the polymeric nanoparticles specifies drug incorporation in the polymeric matrix. The in-vivo study revealed that curcumin-loaded chitosan nanoparticles fasten the healing process of the wound due to the synergistic effect produced by using a combination of curcumin and chitosan. SIGNIFICANCE Curcumin-loaded nanoparticles showed significant enhancement in wound healing action by lowering the dose of curcumin and effecting synergistically due to the use of chitosan.

中文翻译：

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使用天然，合成和半合成聚合物用于伤口愈合的姜黄素纳米颗粒的配制和比较表征。

目的本研究工作旨在使用天然，合成和半合成等不同来源的聚合物制备和表征姜黄素纳米颗粒，并比较其在伤口愈合中的活性。姜黄素是BCS II类药物，是一种具有证明的伤口愈合活性的多酚。材料与方法采用离子凝胶法制备了姜黄素负载的壳聚糖和羧甲基纤维素（CMC）纳米粒子。相反，通过双乳液溶剂蒸发法制备了聚乳酸共乙醇酸（PLGA）纳米颗粒。聚合物的不同来源包括天然（壳聚糖），合成（PLGA）和半合成CMC，用于制备纳米粒子。主要发现载有姜黄素的纳米颗粒的包封率百分数为聚合物PLGA> 壳聚糖> CMC。羧甲基纤维素-姜黄素纳米粒子的体外释放研究发现24小时的释放率为74.96％。在所有聚合物纳米颗粒中姜黄素的特定峰的存在表明药物掺入聚合物基质中。体内研究表明，由于姜黄素和壳聚糖的组合产生协同作用，负载姜黄素的壳聚糖纳米颗粒可加快伤口的愈合过程。意义负载姜黄素的纳米颗粒通过降低姜黄素的剂量并由于使用壳聚糖而协同作用，从而显着增强了伤口的愈合作用。在所有聚合物纳米颗粒中姜黄素的特定峰的存在表明药物掺入聚合物基质中。体内研究表明，由于姜黄素和壳聚糖的组合产生协同作用，负载姜黄素的壳聚糖纳米颗粒可加快伤口的愈合过程。意义负载姜黄素的纳米颗粒由于降低了姜黄素的剂量并由于使用壳聚糖而协同作用，从而显着增强了伤口的愈合作用。在所有聚合物纳米颗粒中姜黄素的特定峰的存在表明药物掺入聚合物基质中。体内研究表明，由于姜黄素和壳聚糖的组合产生协同作用，负载姜黄素的壳聚糖纳米颗粒可加快伤口的愈合过程。意义负载姜黄素的纳米颗粒通过降低姜黄素的剂量并由于使用壳聚糖而协同作用，从而显着增强了伤口的愈合作用。