Spatial Ecology of the Human Tongue Dorsum Microbiome.,Cell Reports

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Spatial Ecology of the Human Tongue Dorsum Microbiome.
Cell Reports ( IF 7.5 ) Pub Date : 2020-03-24 , DOI: 10.1016/j.celrep.2020.02.097
Steven A Wilbert ₁ , Jessica L Mark Welch ₂ , Gary G Borisy ₃

Affiliation

A fundamental question in microbial ecology is how microbes are spatially organized with respect to each other and their host. A test bed for examining this question is the tongue dorsum, which harbors a complex and important microbial community. Here, we use multiplexed fluorescence spectral imaging to investigate the organization of the tongue microbiome at micron to hundred-micron scales. We design oligonucleotide probes for taxa both abundant and prevalent, as determined by sequence analysis. Imaging reveals a highly structured spatial organization of microbial consortia, ranging in linear dimension from tens to hundreds of microns. The consortia appear to develop from a core of epithelial cells, with taxa clustering in domains suggestive of clonal expansion. Quantitative proximity analysis provides the basis for a model of tongue dorsum microbiome organization and dynamics. Our work illustrates how high-resolution analysis of micron-scale organization provides insights into physiological functions and microbiome-host interactions.

中文翻译：

人类舌背微生物组的空间生态学。

微生物生态学中的一个基本问题是微生物如何在空间上相对于彼此及其宿主进行组织。舌背是检验这个问题的试验床，它拥有一个复杂而重要的微生物群落。在这里，我们使用多重荧光光谱成像技术来研究舌头微生物组的组织结构，其微米级至百微米级。我们通过序列分析确定了丰富和普遍的类群的寡核苷酸探针。成像揭示了微生物群落的高度结构化的空间组织，其线性尺寸范围从数十到数百微米。该财团似乎是从上皮细胞的核心发展而来的，在其域中的类群聚集表明克隆的扩张。定量接近分析为舌背微生物组和动力学模型提供了基础。我们的工作说明了微米级组织的高分辨率分析如何提供对生理功能和微生物组-宿主相互作用的见解。

更新日期：2020-03-26

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