Sodium Butyrate Protects the Intestinal Barrier by Modulating Intestinal Host Defense Peptide Expression and Gut Microbiota after a Challenge with Deoxynivalenol in Weaned Piglets,Journal of Agricultural and Food Chemistry

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Sodium Butyrate Protects the Intestinal Barrier by Modulating Intestinal Host Defense Peptide Expression and Gut Microbiota after a Challenge with Deoxynivalenol in Weaned Piglets
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.jafc.0c00791
Shuai Wang ₁ , Cong Zhang ₁ , Jiacheng Yang ₁ , Xu Wang ₁ , Kuntan Wu ₁ , Beiyu Zhang ₁ , Jiacai Zhang ₁ , Ao Yang ₁ , Shahid Ali Rajput ₁ , Desheng Qi ₁

Affiliation

This study aims to determine whether sodium butyrate (SB) could antagonize deoxynivalenol (DON)-induced intestinal epithelial dysfunction. In a four-week feeding trial, twenty-eight barrows were randomly divided into four treatments: (1) uncontaminated basal diet (control); (2) 4 mg/kg DON-contaminated diet (DON); (3) basal diet supplemented with 0.2% SB (SB); and (4) 4 mg/kg DON + 0.2% SB (DON + SB). A decrease in performance was observed in DON-exposed animals, which was prevented by the dietary SB supplementation. DON exposure also depressed the expression of host defense peptides (HDPs) in the intestine, impaired the intestinal barrier integrity, and disturbed the gut microbiota homeostasis. These alterations induced by DON were attenuated by SB supplementation. The supplementation of 0.2% SB ameliorated the adverse effects of DON on the liver in terms of hepatic lesions as well as serum concentrations of alkaline phosphatase and aspartate aminotransferase. In IPEC-J2 cells, pretreatment with SB alleviated the DON-induced decreased cell viability. Additionally, the NOD2/caspase-12 pathway participated in the alleviation of SB on DON-induced diminished HDP expression. Taken together, these data demonstrated that SB protected piglets from DON-induced intestinal barrier dysfunction potentially through stimulation of intestinal HDP assembly and regulation in gut microbiota.

中文翻译：

丁酸钠可通过调节断奶仔猪中脱氧雪腐酚的攻击来调节肠道宿主防御肽的表达和肠道菌群，从而保护肠道屏障。

这项研究旨在确定丁酸钠（SB）是否可以拮抗脱氧雪腐酚（DON）引起的肠上皮功能障碍。在一个为期四周的喂养试验中，将28只手推车随机分为4种处理方法：（1）未经污染的基础饮食（对照）；（2）4 mg / kg受DON污染的饮食（DON）；（3）基础饮食补充0.2％SB（SB）；（4）4 mg / kg DON + 0.2％SB（DON + SB）。在暴露于DON的动物中观察到了性能下降，这是通过饮食中添加SB阻止的。DON暴露还抑制了肠道中宿主防御肽（HDP）的表达，损害了肠道屏障的完整性，并扰乱了肠道菌群的体内稳态。由DON引起的这些改变被SB补充所减弱。补0。2％的SB改善了DON对肝脏损害的肝脏损害以及血清碱性磷酸酶和天冬氨酸转氨酶的浓度。在IPEC-J2细胞中，用SB预处理可减轻DON诱导的细胞活力降低。另外，NOD2 / caspase-12途径参与了DON减轻DON诱导的HDP表达降低的作用。综上所述，这些数据表明，SB可能通过刺激肠道HDP组装和调节肠道菌群来保护仔猪免受DON诱导的肠道屏障功能障碍。NOD2 / caspase-12途径参与了DON减轻DON诱导的HDP表达降低。综上所述，这些数据表明，SB可能通过刺激肠道HDP组装和调节肠道菌群来保护仔猪免受DON诱导的肠道屏障功能障碍。NOD2 / caspase-12途径参与了DON减轻DON诱导的HDP表达降低。综上所述，这些数据表明，SB可能通过刺激肠道HDP组装和调节肠道菌群来保护仔猪免受DON诱导的肠道屏障功能障碍。

更新日期：2020-04-03

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