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Anthocyanin Biosynthesis and Methylation of the MdMYB10 Promoter Are Associated with the Red Blushed-Skin Mutant in the Red Striped-Skin “Changfu 2” Apple
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.jafc.9b07098 Dongjie Jia 1 , Zhenghuan Li 1 , Qingyuan Dang 1 , Lijuan Shang 1 , Junling Shen 1 , Xiangpeng Leng 1 , Yongzhang Wang 1 , Yongbing Yuan 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.jafc.9b07098 Dongjie Jia 1 , Zhenghuan Li 1 , Qingyuan Dang 1 , Lijuan Shang 1 , Junling Shen 1 , Xiangpeng Leng 1 , Yongzhang Wang 1 , Yongbing Yuan 1
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The color of apple skin, particularly anthocyanin-based coloration, is a key factor determining market acceptance. The mechanisms of anthocyanin accumulation in apples with different skin color patterns (i.e., striped and blushed) were analyzed. In total, 14 anthocyanins and 5 procyanidins were simultaneously assayed in red blushed-skin mutants (CF-B1 and CF-B2) and red striped-skin parents (CF-S1 and CF-S2), and 13 significant differences were revealed. Anthocyanin accumulation was significantly higher in the red blushed-skin apples than it was in the parents. The transcript levels of anthocyanin biosynthesis genes and regulatory factors (MdMYB10, MdbHLH3, and MdWD40) were associated with different skin color patterns during the coloring period at 4, 6, and 8 days after the fruits were debagged. The methylation levels of the MdMYB10 promoter regions −1203 to −779 bp, −1667 to −1180 bp, and −2295 to −1929 bp were associated with different skin color patterns, and there was more methylation in red striped-skin apples. These results improve our understanding of anthocyanin accumulation and its underlying molecular mechanism in apples with different skin color patterns, thereby providing valuable information for apple breeding.
中文翻译：
花青素的生物合成和甲基化的MdMYB10启动子与红色条纹皮肤“ Changfu 2”苹果中的红色皮肤突变体相关。
苹果皮的颜色，特别是基于花青素的着色，是决定市场接受度的关键因素。分析了具有不同肤色模式（即条纹和脸红）的苹果中花色苷积累的机理。总共，在红色的红皮肤突变体（CF-B1和CF-B2）和红色的条纹皮肤亲本（CF-S1和CF-S2）中同时检测了14个花色苷和5个原花青素，发现了13个显着差异。红色皮肤红苹果中的花青素积累显着高于其亲本。花青素生物合成基因和调控因子（MdMYB10，MdbHLH3和MdWD40）的转录水平。）在水果脱袋后的第4、6和8天的着色期间与不同的肤色模式相关。MdMYB10启动子区域的−1203至−779 bp，−1667至−1180 bp和−2295至−1929 bp的甲基化水平与不同的皮肤颜色模式相关，在红色条纹皮肤的苹果中甲基化程度更高。这些结果增进了我们对不同肤色图案的苹果中花色苷积累及其潜在分子机制的理解，从而为苹果育种提供了有价值的信息。
更新日期：2020-04-03
中文翻译：
花青素的生物合成和甲基化的MdMYB10启动子与红色条纹皮肤“ Changfu 2”苹果中的红色皮肤突变体相关。
苹果皮的颜色，特别是基于花青素的着色，是决定市场接受度的关键因素。分析了具有不同肤色模式（即条纹和脸红）的苹果中花色苷积累的机理。总共，在红色的红皮肤突变体（CF-B1和CF-B2）和红色的条纹皮肤亲本（CF-S1和CF-S2）中同时检测了14个花色苷和5个原花青素，发现了13个显着差异。红色皮肤红苹果中的花青素积累显着高于其亲本。花青素生物合成基因和调控因子（MdMYB10，MdbHLH3和MdWD40）的转录水平。）在水果脱袋后的第4、6和8天的着色期间与不同的肤色模式相关。MdMYB10启动子区域的−1203至−779 bp，−1667至−1180 bp和−2295至−1929 bp的甲基化水平与不同的皮肤颜色模式相关，在红色条纹皮肤的苹果中甲基化程度更高。这些结果增进了我们对不同肤色图案的苹果中花色苷积累及其潜在分子机制的理解，从而为苹果育种提供了有价值的信息。
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