Versatile Sensing Platform for Cd2+ Detection in Rice Samples and Its Applications in Logic Gate Computation.,Analytical Chemistry

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Versatile Sensing Platform for Cd2+ Detection in Rice Samples and Its Applications in Logic Gate Computation.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-31 , DOI: 10.1021/acs.analchem.0c01022
Junhua Chen _{1,

2} , Jiafeng Pan _{1,

2} , Chengshuai Liu ₃

Affiliation

A versatile sensing platform was designed for Cd2+ detection utilizing Mg2+-dependent DNAzyme as the biocatalyst and toehold-mediated strand replacement as the reaction mechanism. The Cd2+-aptamer interaction brings the split subunits of the Mg2+-dependent DNAzyme into close-enough proximity, which generates an active DNAzyme that can catalyze the cleavage reaction toward the hairpin substrate strand (H1). The trigger DNA fragment in H1 can open another hairpin probe (H2) to activate the cyclic signal amplification process. The generated numerous G-quadruplex DNAzyme structures will produce a high fluorescence response after incubation with the fluorescence dye N-methyl mesoporphyrin IX (NMM). This detection platform is ultrasensitive and the detection limit (LOD) is 2.5 pM (S/N = 3). The sensing system is robust and can work effectively even in a complex sample matrix, enabling the quantitative analysis of Cd2+ content in rice samples with good reliability. Showing the unique features of simple operation, label-free and enzyme-free format, high sensitivity and selectivity, and universal signal amplification mode, our proposed sensing protocol holds great promise for becoming a competitive alternative for the routine monitoring of Cd2+ pollution. Importantly, this flexible and versatile sensing platform was used to construct some exquisite logic gates, including AND, OR, INHIBIT, IMPLICATION, NOR, and NAND.

中文翻译：

水稻样品中Cd2 +检测的多功能传感平台及其在逻辑门计算中的应用。

设计了一个多功能的传感平台，用于检测Cd2 +，利用依赖Mg2 +的DNAzyme作为生物催化剂，并采用脚趾介导的链置换作为反应机制。Cd2 +-适体的相互作用使Mg2 +依赖的DNAzyme的分裂亚基紧密接近，从而产生了一种活性DNAzyme，可以催化向发夹底物链（H1）的裂解反应。H1中的触发DNA片段可以打开另一个发夹探针（H2），以激活循环信号放大过程。与荧光染料N-甲基中卟啉IX（NMM）孵育后，生成的大量G-四链体DNAzyme结构将产生高荧光响应。该检测平台是超灵敏的，检测极限（LOD）为2.5 pM（S / N = 3）。该传感系统坚固耐用，即使在复杂的样品基质中也可以有效地工作，从而可以可靠地定量分析大米样品中Cd2 +的含量。我们提出的传感协议显示出操作简单，无标签和无酶格式，高灵敏度和选择性以及通用信号放大模式的独特功能，因此有望成为常规监测Cd2 +污染的有竞争力的替代方案。重要的是，这个灵活而通用的传感平台被用于构建一些精巧的逻辑门，包括与，或，禁止，隐含，或非和与非。高灵敏度和高选择性，以及通用的信号放大模式，我们提出的传感协议有望成为常规监测Cd2 +污染的有竞争力的替代方案。重要的是，这个灵活而通用的传感平台被用于构建一些精巧的逻辑门，包括与，或，禁止，隐含，或非和与非。高灵敏度和高选择性，以及通用的信号放大模式，我们提出的传感协议有望成为常规监测Cd2 +污染的有竞争力的替代方案。重要的是，这个灵活而通用的传感平台被用于构建一些精巧的逻辑门，包括与，或，禁止，隐含，或非和与非。

更新日期：2020-04-23

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