Carboxylic Acid-Functionalized, Graphitic Layer-Coated Three-Dimensional SERS Substrate for Label-Free Analysis of Alzheimer’s Disease Biomarkers,Nano Letters

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Carboxylic Acid-Functionalized, Graphitic Layer-Coated Three-Dimensional SERS Substrate for Label-Free Analysis of Alzheimer’s Disease Biomarkers
Nano Letters ( IF 9.6 ) Pub Date : 2020-03-24 , DOI: 10.1021/acs.nanolett.0c00048
Hyung Joon Park _{1,

2} , Seunghee Cho ₁ , Minjoon Kim ₁ , Yeon Sik Jung _{1,

2}

Affiliation

Surface-enhanced Raman spectroscopy (SERS)-based protein analysis is a promising alternative to existing early stage diagnoses. However, SERS research conducted thus far accompanies challenges such as nonuniformity of plasmonic nanostructures, irregular coating of analytes, and denaturation of proteins, which seriously limit the practicability of suggested approaches. Here, we introduce a carboxylic acid-functionalized and graphitic nanolayer-coated three-dimensional SERS substrate (CGSS) fabricated by sequential nanotransfer printing. The substrate consists of well-defined, uniform gold nanowire arrays for effective Raman signal enhancement and a strong protein-immobilization layer. With an enhancement factor (EF) of 5.5 × 10⁵, on par with the highest ever reported values, the CGSS allows the detection of protein conformational changes and the determination of protein concentration via Raman measurements. Exploiting the CGSS, we successfully measured the SERS spectra of Alzheimer’s biomarkers, tau protein and amyloid β, based on which secondary structural changes were analyzed quantitatively.

中文翻译：

羧酸功能化，石墨化涂层的三维SERS底物，用于无标记的阿尔茨海默氏病生物标记物分析

基于表面增强拉曼光谱（SERS）的蛋白质分析是现有早期诊断的有希望的替代方法。然而，迄今为止进行的SERS研究伴随着挑战，例如等离子体纳米结构的不均匀性，分析物的不规则包被和蛋白质的变性，这严重限制了所建议方法的实用性。在这里，我们介绍通过顺序纳米转移印刷制造的羧酸功能化和石墨纳米层涂层的三维SERS基板（CGSS）。基质由定义明确的，均匀的金纳米线阵列组成，可有效增强拉曼信号，并具有牢固的蛋白质固定层。增强因子（EF）为5.5×10 ⁵CGSS与有史以来的最高记录值相提并论，可检测蛋白质构象变化并通过拉曼测量确定蛋白质浓度。利用CGSS，我们成功地测量了阿尔茨海默氏症生物标志物，tau蛋白和淀粉样蛋白β的SERS光谱，在此基础上定量分析了二级结构变化。

更新日期：2020-04-24

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