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Correction to Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological Nanopore.
ACS Nano ( IF 17.1 ) Pub Date : 2020-03-24 , DOI: 10.1021/acsnano.0c01699
Laura Restrepo-Pérez , Gang Huang , Peggy R Bohländer , Nathalie Worp , Rienk Eelkema , Giovanni Maglia , Chirlmin Joo , Cees Dekker

In Figure 5c, we reported the correlation between dwell times and electrical charges of tags. We did not account for the sign of some of the tags correctly, leading to an error that we would like to amend. In the published Figure 5c, the value of the parameter charge × P for the labels Alexa 633 and 3PolyA should have had a negative value. Below, we provide a corrected figure and figure legend. We note that this correction does not in any way affect the conclusions of the article. Figure 5. Scatter plots of the peptide labeled with (a) His6 and (b) Alexa633. Faster translocation times are observed in peptides labeled with His6 compared to Alexa633. (c) Plot of dwell time vs net charge × P, where P = M × w/L (see Figure 4). A correlation (R2 = 0.85) is observed between these parameters. The errors in the x-axis for fluorescein, His6, and PEG11 represent the range of values that the parameter can take due to the possible charged states of the molecules. For completeness, we would also like to add some additional citations: (1−3). This article references 3 other publications.

中文翻译:

使用生物纳米孔解决对肽内单个氨基酸化学修饰的化学修饰。

在图5c中,我们报告了停留时间与标签电荷之间的相关性。我们没有正确解释某些标签的符号,从而导致我们要修改的错误。在发布的图5c中,标签Alexa 633和3PolyA的参数charge× P的值应为负值。下面,我们提供了更正的人物和人物图例。我们注意到,此更正不以任何方式影响本文的结论。图5.用(a)His6和(b)Alexa633标记的肽的散点图。与Alexa633相比,在His6标记的肽中观察到了更快的转运时间。(c)停留时间净电荷× P的关系图,其中P = M ×w / L(见图4)。这些参数之间存在相关性(R 2 = 0.85)。荧光素,His6和PEG11在x轴上的误差表示该参数由于分子的可能带电状态而可以取的值的范围。为了完整性,我们还想添加一些其他引用:(1-3)。本文引用了其他3个出版物。
更新日期:2020-03-24
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