Using stem cells to replace the lost beta cells is a hopeful strategy in the treatment of diabetic patients. Furthermore, during stem cell culture and therapy, it is a need to use a substrate to act as a supportive matrix to mimic 3D in vivo microenvironment. Therefore, in this study, human adipose‐derived stem cells were used to differentiate into insulin‐producing cells (IPCs) on a silk/polyethersulfone (PES) scaffold. After exposing to the differentiation media, 2D and 3D (silk/PES) cultured cells were gradually aggregated and formed spherical shaped clusters. The viability of cells was comparable in both 3D and 2D culture. As the results of gene expression assay in both RNA and protein level showed, the differentiation efficiency was higher in 3D culture. Furthermore, ELISA revealed that the release of C‐peptide and insulin was higher in 3D than 2D culture. It seems that silk/PES nanofibrous hybrid scaffold could provide an appropriate matrix to mimic in vivo microenvironment and therefore increases the IPC differentiation potency of stem cells.

中文翻译：

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新型丝绸/ PES杂合纳米纤维支架可促进脂肪来源的间充质干细胞在体外增殖和分化为胰岛素产生细胞

使用干细胞替代丢失的β细胞是治疗糖尿病患者的一种有希望的策略。此外，在干细胞培养和治疗期间，需要使用底物作为模拟3D体内微环境的支持基质。因此，在这项研究中，人类脂肪干细胞被用于在丝/聚醚砜（PES）支架上分化为胰岛素产生细胞（IPC）。暴露于分化培养基后，2D和3D（silk / PES）培养的细胞逐渐聚集并形成球形簇。在3D和2D培养中，细胞的活力均相当。正如在RNA和蛋白质水平上的基因表达分析结果所示，在3D培养物中，分化效率更高。此外，ELISA显示3D中C肽和胰岛素的释放高于2D培养。似乎丝绸/ PES纳米纤维混合支架可以提供一个合适的基质来模拟体内微环境，从而增加干细胞的IPC分化潜能。