Niche-based assembly of bacterial consortia on the diatom Thalassiosira rotula is stable and reproducible.,The ISME Journal

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Niche-based assembly of bacterial consortia on the diatom Thalassiosira rotula is stable and reproducible.
The ISME Journal ( IF 10.8 ) Pub Date : 2020-03-23 , DOI: 10.1038/s41396-020-0631-5
Julian Mönnich ₁ , Jan Tebben ₂ , Jennifer Bergemann ₁ , Rebecca Case _{3,

4} , Sylke Wohlrab _{2,

5} , Tilmann Harder _{1,

2}

Affiliation

With each cell division, phytoplankton create new space for primary colonization by marine bacteria. Although this surface microenvironment is available to all planktonic bacterial colonizers, we show the assembly of bacterial consortia on a cosmopolitan marine diatom to be highly specific and reproducible. While phytoplankton-bacteria interactions play fundamental roles in marine ecosystems, namely primary production and the carbon cycle, the ecological paradigm behind epiphytic microbiome assembly remains poorly understood. In a replicated and repeated primary colonization experiment, we exposed the axenic diatom Thalassiosira rotula to several complex and compositionally different bacterial inocula derived from phytoplankton species of varying degrees of relatedness to the axenic Thalassiosira host or natural seawater. This revealed a convergent assembly of diverse and compositionally different bacterial inocula, containing up to 2071 operational taxonomic units (OTUs), towards a stable and reproducible core community. Four of these OTUs already accounted for a cumulative abundance of 60%. This core community was dominated by Rhodobacteraceae (30.5%), Alteromonadaceae (27.7%), and Oceanospirillales (18.5%) which was qualitatively and quantitatively most similar to its conspecific original. These findings reject a lottery assembly model of bacterial colonization and suggest selective microhabitat filtering. This is likely due to diatom host traits such as surface properties and different levels of specialization resulting in reciprocal stable-state associations.

中文翻译：

硅藻 Thalassiosira rotula 上基于生态位的细菌群落组装是稳定且可重复的。

随着每次细胞分裂，浮游植物为海洋细菌的初级定植创造新的空间。尽管这种表面微环境适用于所有浮游细菌定殖者，但我们表明细菌聚落在大都会海洋硅藻上的组装具有高度特异性和可重复性。虽然浮游植物-细菌相互作用在海洋生态系统（即初级生产和碳循环）中发挥着重要作用，但附生微生物组组装背后的生态范式仍然知之甚少。在重复的初级定殖实验中，我们将纯硅藻轮状海链藻暴露于几种复杂且成分不同的细菌接种物，这些细菌接种物源自与纯海链藻宿主或天然海水有不同程度相关性的浮游植物物种。这揭示了多样化且成分不同的细菌接种物的聚合组装，包含多达 2071 个操作分类单元 (OTU)，形成一个稳定且可重复的核心群落。其中 4 个 OTU 的累积丰度已达到 60%。该核心群落以红杆菌科（30.5%）、交替单胞菌科（27.7%）和海洋螺菌目（18.5%）为主，在质量和数量上与其同种原始群落最为相似。这些发现否定了细菌定植的彩票组装模型，并表明选择性微生境过滤。这可能是由于硅藻宿主特征（例如表面特性）和不同程度的专业化导致了相互的稳定状态关联。

更新日期：2020-03-23

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