Lipases CAL-B, TLL, and RML were used in the synthesis of free fatty acids of grape seed oil as heterogeneous substrate. The best enzyme was used to optimize the reaction variables temperature, enzyme content, and molar ratio of water:oil in batch reactions using experimental planning. The ideal conditions to produce free fatty acids using pure RML were 45 °C, 12:1 substrate molar ratio, and 15% enzyme, resulting in 66% of oil hydrolysis and a productivity of 0.54 mol L⁻¹ min⁻¹ in 4 h of reaction at 180 rpm. Repeated batches of reaction were performed testing the operational stability of RML, results showing that this enzyme could be used for at least 20 cycles keeping more than 80% of its initial activity, suggesting its potential use in industrial processes. The synthesis of free fatty acids was then evaluated in continuous reactions using packed-bed reactor (PBR). The highest productivity in the continuous process was 6.85 mol L⁻¹ min⁻¹, using only RML, showing an operational stability higher than 80% of its initial conversion capacity after 11 days of operation, at a flow rate of 0.13 mL min⁻¹ at 45 °C. We evaluated the use of this hydrolyzed oil as substrate for lactone bioproduction using Galactomyces geotrichum UFMG-CM-Y3276, G. geotrichum UFMG-CM-Y3558, and Geotrichum klebahnii UFMG-CM-Y3014 screened for their oil-hydrolysis ability. Volatile compounds were qualitatively identified in GC–MS as γ-octalactone and γ-nonalactone.

脂肪酶CAL-B，TLL和RML被用于合成葡萄籽油中的游离脂肪酸作为异质底物。最佳酶用于通过实验计划优化间歇反应中的反应变量温度，酶含量和水：油的摩尔比。使用纯RML制备游离脂肪酸的理想条件是45°C，12：1底物摩尔比和15％的酶，导致66％的油水解，生产率为0.54 mol L ^-1  min ^-1在180 rpm下反应4小时。重复进行了一批反应，测试了RML的操作稳定性，结果表明该酶可用于至少20个循环，保持其初始活性的80％以上，表明其在工业过程中的潜在用途。然后使用填充床反应器（PBR）在连续反应中评估游离脂肪酸的合成。仅使用RML，连续过程中的最高生产率为6.85 mol L ^-1  min ^-1，在运行11天后，在0.13 mL min ^-1的流速下，其运行稳定性高于初始转化能力的80％。在45°C下。我们评估了该水解油作为使用乳杆菌的内酯生物生产中底物的用途。筛选了它们的油水解能力：UFMG-CM-Y3276，G。geotrichum UFMG-CM-Y3558和kletahnum klebahnii UFMG-CM-Y3014。在GC-MS中定性鉴定出挥发性化合物为γ-八内酯和γ-九内酯。