Changes in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artificially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteria were chosen for PCR testing, i.e., Bacillus cereus group, Bacillus subtilis group, Pseudomonas sp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identified by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus species represented the most frequently occurring bacterial genus. The presence of the Bacillus subtilis group increased significantly after storage in non-inoculated cabbage heads. The minor part of the other genera identified by sequencing in the first sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.

中文翻译：

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X. campestris pv。储存后细菌谱的变化。campestris接种白菜头（甘蓝变种capitata L.）

在这项研究中，研究了在通常的储存条件下储存后，甘蓝头的细菌光谱变化。用X. campestris pv人工接种甘蓝种子（甘蓝变种capitata L.）。campestris（Xcc），十字花科植物的严重病原体，引起黑腐病。在收获的当天和储存四个月后的两个时间点，从接种Xcc的和未接种的卷心菜头中分离出细菌培养物。根据我们以前的研究和文献报道，选择了细菌的最常见属进行PCR检测，即蜡状芽孢杆菌组，枯草芽孢杆菌组，Pseudomonas sp。和X. campestris pv。桔梗。对于上述四个物种，一些获得的细菌培养物是阴性的。在那些细菌中，其他细菌通过16S rRNA测序鉴定。在Xcc接种和未接种的甘蓝头上，都观察到细菌谱随时间的变化。储存四个月后，头部Xcc感染的严重性增加。芽孢杆菌属是最常见的细菌属。枯草芽孢杆菌的存在储存在未接种的卷心菜头中后，组明显增加。在存储的卷心菜头中未检测到通过第一次采样中的测序鉴定的其他属的一小部分。这与假单胞菌的可能的拮抗行为有关。和芽孢杆菌属。