A bioactive borate glass, 13-93B3 (B3), has been used successfully in the clinic to treat chronic, nonhealing wounds without scarring. However, the mechanism by which B3 stimulates wound healing is poorly understood. Because adipose stem cells (ASCs) have been shown to have multiple roles in wound repair, we hypothesized that B3 triggers ASCs. In this study, we evaluate the effects of B3 on ASC survival, migration, differentiation, and protein secretion in vitro. In concentrations ≤10 mg/ml, B3 did not affect ASC viability under static conditions. B3 promoted the migration of ASCs but did not increase differentiation into bone or fat. B3 also decreased ASCs secretion of collagen I, PAI-1, MCP-1, DR6, DKK-1, angiogenin, IL-1, IGFBP-6, VEGF, and TIMP-2; increased expression of IL-1R and E-selectin; had a transient decrease in IL-6 secretion; and had a transient increase in bFGF secretion. Together, these results show that B3 alters the protein secretion of ASCs.

中文翻译：

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具有生物活性的硼酸盐玻璃触发脂肪干细胞的表型变化。

一种具有生物活性的硼酸盐玻璃13-93B3（B3）已在临床中成功用于治疗慢性，不愈合的伤口而不会留下疤痕。然而，人们对B3刺激伤口愈合的机制了解甚少。由于已显示脂肪干细胞（ASC）在伤口修复中具有多种作用，因此我们假设B3会触发ASC。在这项研究中，我们评估了B3对ASC存活，迁移，分化和蛋白质分泌的影响。浓度≤10mg / ml时，B3在静态条件下不影响ASC的生存力。B3促进了ASC的迁移，但没有增加向骨骼或脂肪的分化。B3还降低了胶原蛋白I，PAI-1，MCP-1，DR6，DKK-1，血管生成素，IL-1，IGFBP-6，VEGF和TIMP-2的ASC分泌；IL-1R和E-选择素的表达增加；IL-6分泌暂时减少；并且bFGF分泌有短暂增加。总之，这些结果表明B3改变了ASC的蛋白质分泌。