In this work, an analytical electrochemical method was developed using a glassy carbon electrode (GCE) for the determination of amiloride (AMI). To study the electrochemical behavior of AMI, the cyclic voltammetry and differential pulse voltammetry techniques were used, adding a AMI solution in 0.1 mol L⁻¹ BR buffer solution (pH 2.0) to the electrochemical cell. A reversible AMI oxi-reduction process was revealed in the scan rate of + 0.4 to − 0.1 V. The DPV parameters optimized were the scan rate (10 mV s⁻¹) and amplitude (70 mV). After parameters optimization, the linear working range was from 9.90 to 90.9 μmol L⁻¹, with detection limit of 5.19 μmol L⁻¹ and quantification limit of 17.3 μmol L⁻¹. The validity of the electroanalytical method for the determination of AMI in biological fluids (urine and serum) was demonstrated. The proposed method is advantageous due to its good sensitivity, speed, simplicity, precision, and accuracy towards AMI quantification.

在这项工作中，开发了一种使用玻碳电极（GCE）测定阿米洛利（AMI）的分析电化学方法。为了研究AMI的电化学行为，使用了循环伏安法和差分脉冲伏安法，将AMI溶液加入0.1 mol L ^-1 BR缓冲溶液（pH 2.0）中，并加入电化学电池。在+ 0.4至-0.1 V的扫描速率下揭示了可逆的AMI氧化还原过程。优化的DPV参数是扫描速率（10 mV s ^-1）和幅度（70 mV）。经过参数优化后，线性工作范围为9.90至90.9μmolL ^-1，检测限为5.19μmolL ^-1，定量限为17.3μmolL ^-1。证明了电分析法测定生物体液（尿液和血清）中AMI的有效性。所提出的方法由于其良好的灵敏度，速度，简便性，精确度以及对AMI定量的准确性而具有优势。