The study investigates the effect of cadmium (Cd), salinity (NaCl), and combined stress on rhizosphere pH, growth parameters, membrane leakage, and genotoxicity in Vicia faba. Germinated seeds were exposed for 48 h to 0.01 mM Cd(NO3)2 (Cd), 50 mM NaCl (S50), 150 mM NaCl (S150), and Cd-NaCl (CdS50 and CdS150). An accumulation of Cd and Na was found essentially in Vicia roots under each single stress factor associated with variations in rhizosphere pH. Additional NaCl in metallic solution significantly dropped the rhizosphere pH and decreased Cd concentrations in roots by 2.3 and 3.8 times for CdS50 and CdS150, respectively. Growth parameters (root length and fresh and dry matters), mitotic activity, and micronucleus formation were not influenced by Cd and low concentration of NaCl when applied separately or together, while 150 mM of NaCl, alone or combined with Cd, affected negatively all the studied parameters, as well as chromosome and nucleus stability. V. faba seems to reduce the transport of Cd in saline conditions and therefore salinity (50 mM) may act as a protection against Cd accumulation.

中文翻译：

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盐度降低了蚕豆中的镉积累。

该研究调查了蚕豆中镉（Cd），盐度（NaCl）和复合胁迫对根际pH，生长参数，膜渗漏和遗传毒性的影响。将发芽的种子暴露于0.01 mM Cd（NO3）2（Cd），50 mM NaCl（S50），150 mM NaCl（S150）和Cd-NaCl（CdS50和CdS150）48小时。在与根际pH值变化相关的每个单一胁迫因子下，基本上在蚕豆根中发现了镉和钠的积累。金属溶液中额外的氯化钠使CdS50和CdS150的根际pH值显着降低，根部的Cd浓度分别降低2.3倍和3.8倍。单独施用或一起施用时，Cd和低浓度的NaCl不会影响生长参数（根长，新鲜和干物质），有丝分裂活性和微核形成，而150 mM的NaCl，单独或与Cd结合使用，会对所有研究参数以及染色体和核稳定性产生负面影响。蚕豆（V. faba）似乎在盐分条件下减少了Cd的运输，因此盐度（50 mM）可以起到防止Cd积累的作用。