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SUMOylation Stabilizes the Transcription Factor DREB2A to Improve Plant Thermotolerance.
Plant Physiology ( IF 6.5 ) Pub Date : 2020-03-23 , DOI: 10.1104/pp.20.00080 Feige Wang 1 , Yiyang Liu 2 , Yaqiao Shi 1 , Danlu Han 1 , Yuanyuan Wu 1 , Weixian Ye 1 , Huanling Yang 3 , Guowei Li 2 , Feng Cui 2 , Shubo Wan 2 , Jianbin Lai 1 , Chengwei Yang 3
Plant Physiology ( IF 6.5 ) Pub Date : 2020-03-23 , DOI: 10.1104/pp.20.00080 Feige Wang 1 , Yiyang Liu 2 , Yaqiao Shi 1 , Danlu Han 1 , Yuanyuan Wu 1 , Weixian Ye 1 , Huanling Yang 3 , Guowei Li 2 , Feng Cui 2 , Shubo Wan 2 , Jianbin Lai 1 , Chengwei Yang 3
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Heat stress (HS) has serious effects on plant development, resulting in heavy agricultural losses. A critical transcription factor network is involved in plant adaptation to high temperature. DEHYDRATION RESPONSIVE ELEMENT-BINDING PROTEIN2A (DREB2A) is a key transcription factor that functions in plant thermotolerance. The DREB2A protein is unstable under normal temperature and is degraded by the 26S proteasome; however, the mechanism by which DREB2A protein stability dramatically increases in response to HS remains poorly understood. In this study, we found that the DREB2A protein of Arabidopsis (Arabidopsis thaliana) is stabilized under high temperature by the posttranslational modification SUMOylation. Biochemical data indicated that DREB2A is SUMOylated at K163, a conserved residue adjacent to the negative regulatory domain during HS. SUMOylation of DREB2A suppresses its interaction with BPM2, a ubiquitin ligase component, consequently increasing DREB2A protein stability under high temperature. In addition, analysis of plant heat tolerance and marker gene expression indicated that DREB2A SUMOylation is essential for its function in the HS response. Collectively, our data reveal a role for SUMOylation in the maintenance of DREB2A stability under high temperature, thus improving our understanding of the regulatory mechanisms underlying HS response in plant cells.
中文翻译：
SUMOylation稳定转录因子DREB2A，以提高植物的耐热性。
热胁迫（HS）对植物发育有严重影响，导致严重的农业损失。关键转录因子网络参与植物对高温的适应。脱水反应性元素结合蛋白2A（DREB2A）是在植物耐热性中起作用的关键转录因子。DREB2A蛋白在常温下不稳定，并被26S蛋白酶体降解；然而，DREB2A蛋白稳定性对HS响应显着增加的机制仍知之甚少。在这项研究中，我们发现拟南芥（Arabidopsis thaliana）的DREB2A蛋白在高温下通过翻译后修饰SUMOylation稳定化。生化数据表明，DREB2A在K163处被SUMO化，这是HS期间与负调控域相邻的保守残基。DREB2A的SUMOylation抑制了它与泛素连接酶成分BPM2的相互作用，因此增加了DREB2A在高温下的蛋白质稳定性。此外，对植物耐热性和标记基因表达的分析表明，DREB2A SUMOylation是其在HS反应中的功能所必需的。总体而言，我们的数据揭示了SUMOylation在高温下维持DREB2A稳定性中的作用，从而增进了我们对植物细胞中HS应答基础调控机制的理解。
更新日期：2020-05-01
中文翻译：
SUMOylation稳定转录因子DREB2A，以提高植物的耐热性。
热胁迫（HS）对植物发育有严重影响，导致严重的农业损失。关键转录因子网络参与植物对高温的适应。脱水反应性元素结合蛋白2A（DREB2A）是在植物耐热性中起作用的关键转录因子。DREB2A蛋白在常温下不稳定，并被26S蛋白酶体降解；然而，DREB2A蛋白稳定性对HS响应显着增加的机制仍知之甚少。在这项研究中，我们发现拟南芥（Arabidopsis thaliana）的DREB2A蛋白在高温下通过翻译后修饰SUMOylation稳定化。生化数据表明，DREB2A在K163处被SUMO化，这是HS期间与负调控域相邻的保守残基。DREB2A的SUMOylation抑制了它与泛素连接酶成分BPM2的相互作用，因此增加了DREB2A在高温下的蛋白质稳定性。此外，对植物耐热性和标记基因表达的分析表明，DREB2A SUMOylation是其在HS反应中的功能所必需的。总体而言，我们的数据揭示了SUMOylation在高温下维持DREB2A稳定性中的作用，从而增进了我们对植物细胞中HS应答基础调控机制的理解。
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