It will be interesting and yet challenging to develop a near-infrared (NIR) laser trigger phototheranostics agent for multimodal imaging guided synergistic phototherapy. In addition, the mechanisms of phototheranostics agent or in combination with NIR for tumor therapy remain unclear. Herein, we fabricate porous Co₃O₄ nanoplates (pCo₃O₄ NPs) using a hydrothermal process followed by a calcination treatment. We demonstrate that pCo₃O₄ NPs are a promising phototheranostics agent for multimodal imaging (photoacoustic/magnetic resonance imaging) and a NIR laser triggered photothermal therapy (PTT) and photodynamic therapy (PDT) of tumors without apparent side effects. Moreover, RNA sequence (RNA-seq) analysis highlights that pCo₃O₄ NPs can suppress tumor metastasis via inhibiting epithelial-mesenchymal transition (EMT) pathway. In addition, pCo₃O₄ NPs could kill tumor cells via inducing DNA damage after NIR irradiation. In summary, the data reveal that pCo₃O₄ NPs + NIR could effectively suppress tumorigenesis, and further highlight their potential to be a nanomaterial-based multimodal imaging contrast agent and precise DNA damage-mediated tumor synergetic phototherapy, providing new insights to fully understand and use phototheranostics agent in the future.

开发用于多模态成像引导的协同光疗的近红外（NIR）激光触发光热疗剂将是有趣且具有挑战性的。此外，光热治疗剂或与NIR结合用于肿瘤治疗的机制仍不清楚。在本文中，我们使用水热工艺然后进行煅烧处理来制造多孔Co ₃ O ₄纳米板（pCo ₃ O ₄ NPs）。我们证明pCo ₃ O ₄NPs是用于多模式成像（光声/磁共振成像）和近红外激光触发的肿瘤的光热疗法（PTT）和光动力疗法（PDT）的有希望的光热疗剂，没有明显的副作用。此外，RNA序列（RNA-seq）分析表明，pCo ₃ O ₄ NPs可通过抑制上皮-间质转化（EMT）途径抑制肿瘤转移。此外，pCo ₃ O ₄ NPs可以通过在NIR照射后诱导DNA损伤来杀死肿瘤细胞。总而言之，数据表明pCo ₃ O ₄ NPs + NIR可以有效抑制肿瘤发生，并进一步凸显其作为基于纳米材料的多峰成像造影剂和精确的DNA损伤介导的肿瘤协同光疗的潜力，为将来全面了解和使用光热疗剂提供新的见解。