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Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process
Applied Energy ( IF 11.2 ) Pub Date : 2020-03-23 , DOI: 10.1016/j.apenergy.2020.114871
Ferdian Wirawan , Chieh-Lun Cheng , Yung-Chung Lo , Chun-Yen Chen , Jo-Shu Chang , Shao-Yuan Leu , Duu-Jong Lee

Bioethanol produced from lignocellulosic materials has been considered as one of the most promising fuels to replace fossil fuels. Immobilized yeasts or bacteria have been frequently used in continuous system due to its feasibility for repeated use with high biomass retention during the continuous process. In this study, continuous SHcF (separate hydrolysis and co-fermentation) and SScF (simultaneous saccharification and co-fermentation) were evaluated for ethanol production from alkaline pretreated sugarcane bagasse using Zymomonas mobilis (PVA immobilized cells) and Pichia stipitis (suspended cells). In SHcF fermentation, the ethanol yield and productivity of 0.36 g ethanol/g cellulose (corresponding to 70.65% of theoretical yield) and 1.868 g/L/h were achieved. In contrast, SScF system resulted in an ethanol yield of 0.414 g ethanol/g cellulose (corresponding to 81.17% of theoretical yield) and ethanol productivity of 0.705 g/L/h. The performance of the two systems are compared and discussed.



中文翻译:

固定化的运动发酵单胞菌和悬浮毕赤酵母在两个阶段的过程中进行纤维素生物乙醇的连续发酵

由木质纤维素材料生产的生物乙醇被认为是替代化石燃料的最有希望的燃料之一。固定化的酵母或细菌由于在连续过程中具有高生物质保留率而可以重复使用的可行性,因此经常在连续系统中使用。在这项研究中,使用运动发酵单胞菌(固定化PVA的细胞)和树干毕赤酵母评价了连续SHcF(单独的水解和共同发酵)和SScF(同时糖化和共同发酵)从碱性预处理甘蔗渣中生产乙醇的能力。(悬浮细胞)。在SHcF发酵中,获得了0.36g乙醇/ g纤维素的乙醇产率和生产率(对应于理论产率的70.65%)和1.868g / L / h。相反,SScF系统产生的乙醇产量为0.414 g乙醇/ g纤维素(相当于理论产量的81.17%),乙醇生产率为0.705 g / L / h。比较和讨论了这两个系统的性能。

更新日期:2020-03-24
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