Nowadays the biological methods based on utilization of bacteria for different purposes in oilfields are becoming more and more popular because these methods potentially may become a cheaper alternative to chemical treatments. Gas hydrates formation in pipelines is the one of the main flow assurance problems. In previous works it has been shown that some biodegraded oils can have components that may act as natural Anti-Agglomerants. These natural Anti-Agglomerants can prevent hydrate particles from agglomeration which allow the particles to flow downstream along with oil in form of slurry. But there is lack of understanding of how products of oil biodegradation affect the kinetic aspects of hydrate nucleation, formation and decomposition. This information might be useful for development of new approached of treatment of the hydrate related issues in oil pipelines.

Impact of the changes in component composition of crude oil caused by biodegradation process on the kinetics of gas hydrates nucleation, formation and decomposition has been studied with different methods. It was demonstrated that the changes of chemical composition of crude oil caused by biodegradation can have a strong impact on the hydrate nucleation rates at static conditions. Almost twofold decrease of hydrate nucleation rate was observed in the experiments with the water-in-oil emulsion for the biodegraded oils compared to one obtained with emulsion in the original crude oil. At the same time, no significant effect of biodegradation on the hydrate film growth rates at static conditions was observed. Similar tangential and normal hydrate film growth rates were obtained in experiments with both crude oil and with biodegraded oil. Initial growth rates of hydrate in stirring emulsions were an order of magnitude higher for water emulsions in the biodegraded oil compared to ones for the original crude oil. The studies of the decomposition rates of hydrates at sub-zero temperatures and at dynamic conditions showed that the products of vital activity of bacteria promoted self-preservation of hydrates dispersed in the biodegraded oil.

如今，基于细菌在油田中用于不同目的的生物方法正变得越来越流行，因为这些方法可能会成为化学处理的一种廉价替代品。管道中天然气水合物的形成是主要的流量保证问题之一。在以前的工作中，已经表明一些生物降解的油可能具有某些成分，可以用作天然的抗结块剂。这些天然的抗结块剂可以防止水合物颗粒聚结，使水合物颗粒与油一起以浆状形式向下游流动。但是，人们缺乏对石油生物降解产物如何影响水合物成核，形成和分解动力学方面的认识。

通过不同的方法研究了生物降解过程引起的原油成分组成变化对天然气水合物成核，形成和分解动力学的影响。结果表明，在静态条件下，生物降解引起的原油化学成分变化对水合物成核速率有很大影响。与原始原油中的乳液得到的油包水乳液相比，在生物降解油的油包水乳液的实验中观察到水合物成核速率几乎降低了两倍。同时，在静态条件下未观察到生物降解对水合物膜生长速率的显着影响。在原油和生物降解油的实验中均获得了相似的切向和正常水合物膜生长速率。与原始原油相比，生物降解油中水乳液在搅拌乳液中的初始生长速率高一个数量级。水合物在零度以下温度和动态条件下的分解速率研究表明，细菌的生命活动产物促进了分散在生物降解油中的水合物的自我保存。